CAJ | 학술논문

目的:体外研究肺癌细胞条件培养液(conditioned medium,CM)对前骨细胞亚克隆14细胞株MC3T3-E1 Subclone 14增殖、分化和矿化的作用.方法:收集人肺腺癌A549细胞CM,以10％ A549 CM和20％ A549 CM加入MC3T3-E1 Subclone 14细胞培养液,采用CCK-8(cell counting kit-8)法检测MC3T3-E1Subclone 14细胞增殖,碱性磷酸酶(alkaline phosphatase,ALP)试剂盒检测MC3T3-E1 Subclone 14细胞中ALP的活性,茜素红染色检测MC3T3-E1 Subclone 14细胞的矿化能力,实时荧光定量PCR检测MC3T3-E1Subclone 14细胞中ALP、骨钙蛋白(osteocalcin,OCN)、Ⅰ型胶原蛋白α1 (collagen type Ⅰ alpha 1,Col1α1)及RUNX2 (runt-related transcription factor 2) mRNA的表达.结果:MC3T3-E1 Subclone 14细胞培养24和48 h时,10％ A549 CM组和20％ A549 CM组的细胞增殖率均高于不加A549 CM的对照组(P＜0.05); 10％ A549CM组和20％ A549 CM组MC3T3-E1 Subclone 14细胞中ALP活性低于对照组(P＜0.05); A549 CM组矿化结节的数量和面积均小于对照组(P＜0.05); A549CM组MC3T3-E1 Subclone 14细胞中ALP、OCN、Col1α1和RUNX2 mRNAs的表达水平明显低于对照组(P＜0.05).结论:肺癌A549细胞CM可促进MC3T3-E1Subclone 14细胞的增殖,抑制其分化和矿化.
목적:체외연구폐암세포조건배양액(conditioned medium,CM)대전골세포아극륭14세포주MC3T3-E1 Subclone 14증식、분화화광화적작용.방법:수집인폐선암A549세포CM,이10％ A549 CM화20％ A549 CM가입MC3T3-E1 Subclone 14세포배양액,채용CCK-8(cell counting kit-8)법검측MC3T3-E1Subclone 14세포증식,감성린산매(alkaline phosphatase,ALP)시제합검측MC3T3-E1 Subclone 14세포중ALP적활성,천소홍염색검측MC3T3-E1 Subclone 14세포적광화능력,실시형광정량PCR검측MC3T3-E1Subclone 14세포중ALP、골개단백(osteocalcin,OCN)、Ⅰ형효원단백α1 (collagen type Ⅰ alpha 1,Col1α1)급RUNX2 (runt-related transcription factor 2) mRNA적표체.결과:MC3T3-E1 Subclone 14세포배양24화48 h시,10％ A549 CM조화20％ A549 CM조적세포증식솔균고우불가A549 CM적대조조(P＜0.05); 10％ A549CM조화20％ A549 CM조MC3T3-E1 Subclone 14세포중ALP활성저우대조조(P＜0.05); A549 CM조광화결절적수량화면적균소우대조조(P＜0.05); A549CM조MC3T3-E1 Subclone 14세포중ALP、OCN、Col1α1화RUNX2 mRNAs적표체수평명현저우대조조(P＜0.05).결론:폐암A549세포CM가촉진MC3T3-E1Subclone 14세포적증식,억제기분화화광화.