CAJ | 학술논문

目的：比较CCK-8法与BrdU-ELISA法检测人肝癌HepG2细胞增殖的可靠性。方法待细胞贴壁生长融合度分别达60％及80％时，以10-7mol/L浓度AngⅡ刺激细胞增殖，24小时后分别用CCK-8法和BrdU-ELISA法，以及免疫荧光方法检测细胞增殖情况。结果细胞60％汇合时CCK-8检测组和BrdU-ELISA检测组与各自的对照组比较，比值分别为1.30和1.59，检测值近似；细胞80％汇合时二者分别为1.02和1.40，免疫荧光结果显示AngⅡ刺激组的增殖细胞明显高于对照组。结论与CCK-8法相比，BrdU-ELISA更能客观地反映细胞增殖情况。
목적：비교CCK-8법여BrdU-ELISA법검측인간암HepG2세포증식적가고성。방법대세포첩벽생장융합도분별체60％급80％시，이10-7mol/L농도AngⅡ자격세포증식，24소시후분별용CCK-8법화BrdU-ELISA법，이급면역형광방법검측세포증식정황。결과세포60％회합시CCK-8검측조화BrdU-ELISA검측조여각자적대조조비교，비치분별위1.30화1.59，검측치근사；세포80％회합시이자분별위1.02화1.40，면역형광결과현시AngⅡ자격조적증식세포명현고우대조조。결론여CCK-8법상비，BrdU-ELISA경능객관지반영세포증식정황。
Objective To compare the reliability between CCK-8 method and BrdU ELISA method in measure the proliferation of human hepatic cancer cell lines HepG2. Methods Perform CCK-8 and BrdU-ELISA method, as well as immunofluorescence method to examine the the proliferation of cells stimulated by using Ang II with concentration of 10-7mol/L 24 hours when the degree of the cell confluence reaches 60%and 80%. Results The ratio are respectively 1.30 and 1.59 comparing with their own control group respectively by CCK-8 and BrdU-ELISA method under the circumstance that the cell confluence reaches 60%which have an approximate estimated value;and the ratio are 1.02 and 1.40 when the cell confluence reaches 80%which the immunofluorescence method reveals that those of the group with stimulation of Ang II have a obviously higher cell proliferation than control group. Conclusion BrdU-ELISA method could present a more objective situation of the cell proliferation compared with CCK-8 method.