CAJ | 학술논문

利用简单钙溶液培养法对广东高州普通野生稻部分编号进行了耐铝性鉴定，对盆栽条件下低 pH 值和 Al胁迫对植株重要农艺性状的影响进行了调查，并利用铝敏感品种华粳籼74为受体与高州普通野生稻耐铝性编号为供体所构建的高世代回交群体 BC3 F3对高州普通野生稻耐铝性 QTL 进行了初步定位分析。结果表明，在铝处理浓度为50μmol/L 的0．5 mmol /L CaCl2（pH 值＝4．5）溶液培养24 h 后，供试的8个野生稻材料种子根的相对根伸长量RRE 均大于0．5；以其中4个编号的离蘖茎新生根在相同胁迫条件下培养，发现其仍然表现 RRE ＞0．5。表明供试野生稻具有耐铝性。盆栽试验结果表明，Al 胁迫对野生稻和栽培稻植株株高影响明显，处理材料的株高明显低于对照，结实率和千粒质量也受到 Al 胁迫的不利影响。利用以华粳籼74为受体与供试野生稻构建的 BC3 F3群体，运用基于混合线性模型的复合区间作图法（MCIM）进行 QTL 分析，共鉴定到4个耐铝毒 QTL。其中 qRRE－6－2与 qRRE－7－2来自华粳籼74×GZW003的 BC3 F3群体，qRRE－4与 qRRE－2－2则分别来自华粳籼74×GZW006及华粳籼74×GZW087的BC3 F3群体。这4个 QTL 分别解释了表型遗传变异的18．33％，9．18％，19．02％，24．88％；除 qRRE－6－2外，其余3个耐铝性增效基因来均自供体亲本高州普通野生稻。
이용간단개용액배양법대엄동고주보통야생도부분편호진행료내려성감정，대분재조건하저 pH 치화 Al협박대식주중요농예성상적영향진행료조사，병이용려민감품충화갱선74위수체여고주보통야생도내려성편호위공체소구건적고세대회교군체 BC3 F3대고주보통야생도내려성 QTL 진행료초보정위분석。결과표명，재려처리농도위50μmol/L 적0．5 mmol /L CaCl2（pH 치＝4．5）용액배양24 h 후，공시적8개야생도재료충자근적상대근신장량RRE 균대우0．5；이기중4개편호적리얼경신생근재상동협박조건하배양，발현기잉연표현 RRE ＞0．5。표명공시야생도구유내려성。분재시험결과표명，Al 협박대야생도화재배도식주주고영향명현，처리재료적주고명현저우대조，결실솔화천립질량야수도 Al 협박적불리영향。이용이화갱선74위수체여공시야생도구건적 BC3 F3군체，운용기우혼합선성모형적복합구간작도법（MCIM）진행 QTL 분석，공감정도4개내려독 QTL。기중 qRRE－6－2여 qRRE－7－2래자화갱선74×GZW003적 BC3 F3군체，qRRE－4여 qRRE－2－2칙분별래자화갱선74×GZW006급화갱선74×GZW087적BC3 F3군체。저4개 QTL 분별해석료표형유전변이적18．33％，9．18％，19．02％，24．88％；제 qRRE－6－2외，기여3개내려성증효기인래균자공체친본고주보통야생도。
@@@@In this study,the tolerance to aluminum of eight accessions of O.rufipogon indigenous to Gaozhouwas identified by culturing in simple CaCl2 solution,and some important agronomic traits of rice plants cultured inpot soils with low pH or adding Al were investigated ,Al-tolerance related QTLs were also mapped primarily by usingthe advanced backcross population BC 3 F3 between Huajingxian 74 and accessions of O.rufipogon indigenous toGaozhou.The results were as follow.After seedling roots of O.rufipogon were treated in 0.5 mmol/L CaCl2 solutionwith 50 μmol/L Al3 +(pH =4.5) for 24 h,the relative root elongation (RRE) of eight accessions were all higherthan 0.5.Meanwhile,RRE of the regenerative roots from tillerings of the four out of the eight accessions also higherthan 0.5.It is considered that the experimental accessions of O.rufipogon were Al tolerant.Investigation of planttraits in the pot soil cultured experiments showed that the rice plant height was obviously affected by Al stress ,alsoas seed setting rate and 1000- grain weight.Four QTLs related to Al-tolerance were mapped in BC3 F3 population bythe method of MCIM.Hereinto,qRRE-6-2 and qRRE-7-2,which were mapped in BC3 F3 population derived fromHuajingxian 74 ×GZW003,qRRE-4 and qRRE-2-2,which were mapped in BC3 F3 population derived from Huajingxian74 ×GZW006 and Huajingxian 74 ×GZW087,respectively.These four QTLs explained 18.33%,9.18%,19.02% and 24.88% of the phenotypic variance,respectively.Except of qRRE-6-2,the favorable alleles of the other three QTLs were all derived from the donor parent ,O.rufipogon.