CAJ | 학술논문

长叶红砂为内蒙古东阿拉善－西鄂尔多斯地区特有珍稀泌盐，强旱生小灌木，对盐渍荒漠环境具有极强适应性。利用 RT－PCR 和 RACE 技术从该植物中分离出质膜 Na＋/H＋逆向转运蛋白基因（RtSOS1），该 cDNA 全长为3829 bp，开放阅读框为3438 bp，编码一个含1145个氨基酸的蛋白质，推测分子量为126.76 kDa。氨基酸序列的生物信息学分析推测，该蛋白 N 端含有11个跨膜结构域，C 端为一个长约700个氨基酸的亲水性尾，具有磷酸化和自我抑制结构域。同源性比对和系统发育分析证实，RtSOS1与其他植物的质膜 Na＋/H＋逆向转运蛋白亲缘关系较近。
장협홍사위내몽고동아랍선－서악이다사지구특유진희비염，강한생소관목，대염지황막배경구유겁강괄응성。이용 RT－PCR 화 RACE 기술종해식물중분리출질막 Na＋/H＋역향전운단백기인（RtSOS1），해 cDNA 전장위3829 bp，개방열독광위3438 bp，편마일개함1145개안기산적단백질，추측분자량위126.76 kDa。안기산서렬적생물신식학분석추측，해단백 N 단함유11개과막결구역，C 단위일개장약700개안기산적친수성미，구유린산화화자아억제결구역。동원성비대화계통발육분석증실，RtSOS1여기타식물적질막 Na＋/H＋역향전운단백친연관계교근。
Reaumuria trigyna Maxim.is an endangered small shrub with the features of a recretohalophyte .This species is endemic to the Eastern Alxa Western Ordos area and developed distinctive strategies to adapt to the semi -desert and salty soil environment .A full-length cDNA of the plasma Na+/H+antiporter (RtSOS1) was isolated from this species by using RT-PCR and RACE technologies.The 3 829 bp sequence comprised a 3 438 bp open reading frame,encoding an 1 145 amino acids protein with the molecular weight of 126.76 kDa.Bioinformatics analyze re-veals that RtSOS1 composed of 11 transmembrane domains within its N terminal portion ,and a hydrophilic cytoplas-mic tail with the length approximately 700 amino acids in its C-terminal portion.In the C-terminal region,the phos-phorylation domain and the auto -inhibited domain are found.The Homology comparison and phylogenetic analysis showed that RtSOS1 is related to plasma membrane Na+/H+antiporter in other plant species.