CAJ | 학술논문

为分析系列化学诱变对玉米自交系 AS－9诱变后代的影响，以玉米自交系 AS－9及其化学诱变自交第1代M1和第4代 M4为材料，进行发芽试验，同时利用分布在10条染色体上的68对 SSR 引物进行遗传变异分析。结果表明：诱变 M1和 M4种子的发芽率、芽长和根数普遍高于对照；能在2代玉米试材中扩增出多态性条带的引物有54对，占引物总数的79．41％；M1诱变系与基础材料对照的遗传相似系数平均值为0．3647，遗传相似系数中心化后的数据在－0．12处，可将材料分为2个类群，基础材料（ⅠCK）为一类，诱变系为一类； M4诱变系与基础材料对照的遗传相似系数平均值为0．4346，遗传相似系数中心化后的数据在－0．09处，可将材料分为基础自交系（ⅣCK）和诱变系2个类群；M1和 M4主成分分析与聚类结果基本一致。说明基础材料 AS－9与诱变系材料间存在真实的遗传差异，系列化学诱变已使玉米自交系 AS－9产生了广泛的遗传变异。
위분석계렬화학유변대옥미자교계 AS－9유변후대적영향，이옥미자교계 AS－9급기화학유변자교제1대M1화제4대 M4위재료，진행발아시험，동시이용분포재10조염색체상적68대 SSR 인물진행유전변이분석。결과표명：유변 M1화 M4충자적발아솔、아장화근수보편고우대조；능재2대옥미시재중확증출다태성조대적인물유54대，점인물총수적79．41％；M1유변계여기출재료대조적유전상사계수평균치위0．3647，유전상사계수중심화후적수거재－0．12처，가장재료분위2개류군，기출재료（ⅠCK）위일류，유변계위일류； M4유변계여기출재료대조적유전상사계수평균치위0．4346，유전상사계수중심화후적수거재－0．09처，가장재료분위기출자교계（ⅣCK）화유변계2개류군；M1화 M4주성분분석여취류결과기본일치。설명기출재료 AS－9여유변계재료간존재진실적유전차이，계렬화학유변이사옥미자교계 AS－9산생료엄범적유전변이。
Analysis of genetic diversity in maize germplasm is of great importance to conserving and utilizing these valuable chemical mutagenic resources .In this research,the genetic differences between mutants (M1&M4) and the corresponding orginal material (AS-9) was studied based on SSR markers with selected 54 polymorphism SSR primers.The results showed that the seed germination rate ,bud length and root number of M1 &M4 were higher than AS-9 in most cases.In two generations maize materials,54 polymorphism primers can be amplified,accounting for 79.41% of the total.Relatived toⅠCK ,the average value of genetic similarity coefficient of M1 was 0.364 7. With the certered genetic similarity coefficient of -0.12 by UPGMA,the maize inbred line materials were divided into two groups:the orginal material(ⅠCK) and mutants.Relatived to ⅣCK,the average value of genetic similarity coefficient of M4 was 0.434 6.With the cemtered genetic similarity coefficient of -0.09,the maize materials were classified into two groups:ⅣCK and mutants.Principal component analysis and clustering results were similar .The results showed there were distinct variation between orginal material AS -9 and induced material,indicating chemical mutagen had produced genetic variations in maize inbred lines on large scale .