CAJ | 학술논문

以不同生态型甘蓝抗枯萎病高代自交系 R4－P1和感枯萎病自交系 R2－P2组配得到的142个 F2单株作为构图群体，利用 AFLP、SSR、SRAP 这3种分子标记技术对该群体进行遗传连锁分析，构建了一张含有9个连锁群、240个标记位点的高密度甘蓝分子遗传连锁图谱（LOD≥4），其包含162个 AFLP 标记、52个 SSR 标记和26个 SRAP 标记，标记间平均图距3．6 cM，覆盖864．4 cM。该图谱涉及标记种类多，是目前发表的覆盖基因组较全面的结球甘蓝亚种内遗传连锁图谱，可供参考的 SSR 标记数量多，将为后期甘蓝枯萎病抗性基因的定位及克隆搭建良好平台，同时为结球甘蓝重要农艺性状的 QTL 定位及分子标记辅助选育新品种奠定基础。
이불동생태형감람항고위병고대자교계 R4－P1화감고위병자교계 R2－P2조배득도적142개 F2단주작위구도군체，이용 AFLP、SSR、SRAP 저3충분자표기기술대해군체진행유전련쇄분석，구건료일장함유9개련쇄군、240개표기위점적고밀도감람분자유전련쇄도보（LOD≥4），기포함162개 AFLP 표기、52개 SSR 표기화26개 SRAP 표기，표기간평균도거3．6 cM，복개864．4 cM。해도보섭급표기충류다，시목전발표적복개기인조교전면적결구감람아충내유전련쇄도보，가공삼고적 SSR 표기수량다，장위후기감람고위병항성기인적정위급극륭탑건량호평태，동시위결구감람중요농예성상적 QTL 정위급분자표기보조선육신품충전정기출。
A F2 population consisting of 142 individual plants derived from a cross between fusarium wilt resistant cabbage self line R4-P1 and fusarium wilt susceptible line R2-P2 combining with AFLP ,SSR and SRAP markers were applied to construct a high density genetic linkage map of Brassica oleracea.In total,240 markers including 162 AFLP markers,52 SSR markers and 26 SRAP markers were integrated into 9 groups(LOD≥4).It covered 864.4 cM,with the average interval of 3.6 cM.It is a relatively high dense map involving more kinds of markers especially more refer -ence SSR markers.It is also one of the most comprehensive intrasific genetic linkage map of covering whole genome of cabbage.It will build a good platform for mapping and cloning of the fusarium wilt resistant gene in cabbage ,and fur-thermore,lay the foundation for mapping important agronomic traits for marker assistant breeding .