CAJ | 학술논문

小麦品系 L224－3苗期和成株期对目前大多叶锈菌生理小种表现抗性，鉴定和定位其中抗叶锈病基因对培育持久抗叶锈品种十分重要。苗期利用 L224－3及37携带已知抗叶锈病基因的对照品系接种15个中国小麦叶锈菌小种，同时对由 L224－3和感病品种郑州5389杂交获得的 F2：3群体进行抗叶锈性遗传分析，进一步利用 SSR 和 STS 标记对 L224－3中的抗叶锈病基因进行分子定位。遗传分析结果显示，L224－3可能携带1对显性抗病基因，暂命名为LrL224，标记分析表明，LrL224与1BL 上的4个 SSR 标记 barc8、gwm582、wmc419、wmc694和1个 STS 标记（ω－secali /Glu－B3）紧密连锁，其中距离最近的2个 SSR 标记为 barc8、gwm582，与 LrL224的遗传距离分别为4．3，4．6 cM。 LrL224位于1BL 染色体上，鉴于之前对其他已知抗叶锈病基因的研究，其抗性反应均与此不同，推断其可能为1个新的抗叶锈病基因。
소맥품계 L224－3묘기화성주기대목전대다협수균생리소충표현항성，감정화정위기중항협수병기인대배육지구항협수품충십분중요。묘기이용 L224－3급37휴대이지항협수병기인적대조품계접충15개중국소맥협수균소충，동시대유 L224－3화감병품충정주5389잡교획득적 F2：3군체진행항협수성유전분석，진일보이용 SSR 화 STS 표기대 L224－3중적항협수병기인진행분자정위。유전분석결과현시，L224－3가능휴대1대현성항병기인，잠명명위LrL224，표기분석표명，LrL224여1BL 상적4개 SSR 표기 barc8、gwm582、wmc419、wmc694화1개 STS 표기（ω－secali /Glu－B3）긴밀련쇄，기중거리최근적2개 SSR 표기위 barc8、gwm582，여 LrL224적유전거리분별위4．3，4．6 cM。 LrL224위우1BL 염색체상，감우지전대기타이지항협수병기인적연구，기항성반응균여차불동，추단기가능위1개신적항협수병기인。
Wheat line L224-3 showed high resistance to most of Puccinia triticina pathotypes in the seedling and adult stage in the field.Identify and map the leaf rust resistance gene (s) in L224-3 is very useful to breed wheat cultivars with durable resistance to leaf rust .L224-3 and 37 check lines with known leaf rust resistance genes were inoculated with 15 pathotypes at seedling stage to postulate gene in L224-3.F2:3 population from the cross L224-3 /Zhengzhou 5389 were identified with pathotype FHDQ for genetic analysis of leaf rust resistance in L 224-3. SSR and STS markers were used to map the gene in L224-3.L224-3 might contain a dominant resistance gene ,tem-porarily designated LrL224.Totally four SSR markers (barc8,gwm582,wmc419,wmc694) and one STS marker (ω-secali /Glu-B3) on 1B were closely linked to LrL224.The two flanking SSR loci were barc8 and gwm582,with the genetic distances of 4.3 and 4.6 cM,respectively.LrL224 was located on 1BL,and it showed different seedling re -action with other genes on 1B.Therefore LrL224 is likely to be a new leaf rust resistance gene .