CAJ | 학술논문

小麦 TaNADP－ME2是一光反应基因，并对干旱、盐、低温等非生物胁迫作出响应。构建 TaNADP－ME2基因的重组植物表达载体，并转化水稻获得转基因植株。利用重组技术构建植物表达载体，冻融法转化农杆菌，农杆菌介导方法转化水稻成熟胚诱导的愈伤组织，潮霉素筛选和 PCR 鉴定转基因植株。成功构建了重组植物表达质粒pSUE2，并将 pSUE2转入农杆菌 EHA105，潮霉素磷酸转移酶（HPT）基因和 TaNADP－ME2基因 PCR 鉴定获得16棵转基因阳性水稻植株。为进一步确定 TaNADP－ME2基因的水分利用效率功能奠定了基础。
소맥 TaNADP－ME2시일광반응기인，병대간한、염、저온등비생물협박작출향응。구건 TaNADP－ME2기인적중조식물표체재체，병전화수도획득전기인식주。이용중조기술구건식물표체재체，동융법전화농간균，농간균개도방법전화수도성숙배유도적유상조직，조매소사선화 PCR 감정전기인식주。성공구건료중조식물표체질립pSUE2，병장 pSUE2전입농간균 EHA105，조매소린산전이매（HPT）기인화 TaNADP－ME2기인 PCR 감정획득16과전기인양성수도식주。위진일보학정 TaNADP－ME2기인적수분이용효솔공능전정료기출。
The wheat TaNADP-ME2 was not only responsive to light but also to drought ,salt and low tempera-ture.The recombinant plant overexpression vector was constructed ,transformed into rice and acquired transgenic