CAJ | 학술논문

基于物种同源性，应用 PCR 和 RT－PCR 技术，克隆得到藏山羊瓣胃溶菌酶基因 cDNA 编码序列，并命名为TGOLyz，应用生物信息学软件对 TGOLyz 基因核苷酸序列及预测的蛋白序列进行了分析，并预测该蛋白的三级结构；应用 qRT－PCR 技术检测了 TGOLyz 在5个组织中的定量表达。结果表明，藏山羊瓣胃溶菌酶基因 TGOLyz cDNA 编码区长444 bp（Accession No．KC558501），编码147个氨基酸，分子量为16．29 kDa，等电点为6．08。同源性对比显示， TGOLyz 与牛胃1（Cow stomach 1）的同源性最高，达95．24％，与牦牛胃（Yak stomach）的同源性最低，仅为67．61％；系统进化树显示，该基因属于 c 型溶菌酶（c－type lysozyme）家族；qRT－PCR 分析表明，TGOLyz 基因在5个组织中均有表达，在瓣胃中表达量最高。这为进一步深入研究 TGOLyz 的抗菌及消化功能的分子调控机制提供了理论基础。
기우물충동원성，응용 PCR 화 RT－PCR 기술，극륭득도장산양판위용균매기인 cDNA 편마서렬，병명명위TGOLyz，응용생물신식학연건대 TGOLyz 기인핵감산서렬급예측적단백서렬진행료분석，병예측해단백적삼급결구；응용 qRT－PCR 기술검측료 TGOLyz 재5개조직중적정량표체。결과표명，장산양판위용균매기인 TGOLyz cDNA 편마구장444 bp（Accession No．KC558501），편마147개안기산，분자량위16．29 kDa，등전점위6．08。동원성대비현시， TGOLyz 여우위1（Cow stomach 1）적동원성최고，체95．24％，여모우위（Yak stomach）적동원성최저，부위67．61％；계통진화수현시，해기인속우 c 형용균매（c－type lysozyme）가족；qRT－PCR 분석표명，TGOLyz 기인재5개조직중균유표체，재판위중표체량최고。저위진일보심입연구 TGOLyz 적항균급소화공능적분자조공궤제제공료이론기출。
Based on species homology,the lysozyme cDNA was cloned from omasum in Tibetan goat by PCR and RT-PCR,and named TGOLyz gene.The sequence of nucleotides and deduced amino acids of TGOLyz was ana-lyzed through bioinformatics methods ,and the gene expression levels at five tissues of Tibetan goat were detected through qRT-PCR.The result showed that the coding region of the TGOLyz cDNA was 444 bp,and encoded 147 a-mino acids with a molecular weight of 16.29 kDa and isoelectric point of 6.08.The deduced amino acids sequence of TGOLyz shared 95.24% homologies with that from cow stomach 1 and had 67.61% homologies with that from yak stomach.The phylogenetic analysis showed that the TGOLyz gene belongs to c-type lysozyme.Real-time quanti-tative PCR assay demonstrated that the TGOLyz could be detected in all 5 tissues with the highest expression level observed in omasum.All these would establish a foundation for TGOLyz of its molecular mechanism of antibacterial and digestive function.