CAJ | 학술논문

利用筛选和验证相结合的方法筛选出了产碱性脂肪酶活性较高的菌株BL1011。经过形态观察、生理生化试验及分子生物学鉴定，结果表明该菌株为假单胞菌（Pseudomonas sp．）。运用单因素试验和均匀试验优化了BL1011菌株摇瓶产酶培养基和最佳发酵条件。在培养基为麦芽糖2．5％，蛋白胨3．0％，大豆油0．5％，K2HPO40．2％，培养条件为起始pH值7．5，温度33℃，转速180r／min，装液量20mL，发酵周期为60h的条件下，酶活力达到最高，为223U／mL。
이용사선화험증상결합적방법사선출료산감성지방매활성교고적균주BL1011。경과형태관찰、생리생화시험급분자생물학감정，결과표명해균주위가단포균（Pseudomonas sp．）。운용단인소시험화균균시험우화료BL1011균주요병산매배양기화최가발효조건。재배양기위맥아당2．5％，단백동3．0％，대두유0．5％，K2HPO40．2％，배양조건위기시pH치7．5，온도33℃，전속180r／min，장액량20mL，발효주기위60h적조건하，매활력체도최고，위223U／mL。
An alkaline lipase-producing strain BL1011 was screened quickly through the screening and confirmation.According to morphologic observation ,physiology biochemical experiment and molecular identification ,this strain was Pseuclornonas sp.Using single factor and uniform design approaches, the best fermentation medium and fermentation condition were optimized. The optimized culture medium was maltose 2.5%, peptone 3.0%, soya-bean oil 0.5% ,K2HPO4 0.2% .The initiative pH,temperature,agitation speed and medium volume were 7.5,33 ℃ ,180 dmin and 20 mL, respectively. The highest enzyme activity was 223 U/mL after 60 hours.