现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2012年
22期
154-154,158
,共2页
张俊%邱艳红%胡庆%尹斌开
張俊%邱豔紅%鬍慶%尹斌開
장준%구염홍%호경%윤빈개
荷兰铁%组织培养%技术研究
荷蘭鐵%組織培養%技術研究
하란철%조직배양%기술연구
Yucca elephantipes%tissue culture%technology research
对荷兰铁组织培养方法进行研究,结果表明:荷兰铁顶芽经消毒处理后接种到MS+6-BA2.0mg/L+NAA0.1mg/L培养基中,可诱导出愈伤组织和不定芽:在1/2MS+6-BA1.0mg/L+NAA0.1mg/L培养基中继代培养,扩增倍数(MR值)约2.2倍;将继代培养芽丛中高3cm以上的不定芽接种到生根培养基1/2MS+IAA0.3mg/L中,30d左右生根率达98.2%;炼苗20-30d后移栽,成活率迭95%以上。
對荷蘭鐵組織培養方法進行研究,結果錶明:荷蘭鐵頂芽經消毒處理後接種到MS+6-BA2.0mg/L+NAA0.1mg/L培養基中,可誘導齣愈傷組織和不定芽:在1/2MS+6-BA1.0mg/L+NAA0.1mg/L培養基中繼代培養,擴增倍數(MR值)約2.2倍;將繼代培養芽叢中高3cm以上的不定芽接種到生根培養基1/2MS+IAA0.3mg/L中,30d左右生根率達98.2%;煉苗20-30d後移栽,成活率迭95%以上。
대하란철조직배양방법진행연구,결과표명:하란철정아경소독처리후접충도MS+6-BA2.0mg/L+NAA0.1mg/L배양기중,가유도출유상조직화불정아:재1/2MS+6-BA1.0mg/L+NAA0.1mg/L배양기중계대배양,확증배수(MR치)약2.2배;장계대배양아총중고3cm이상적불정아접충도생근배양기1/2MS+IAA0.3mg/L중,30d좌우생근솔체98.2%;련묘20-30d후이재,성활솔질95%이상。
Abstract The tissue culture of Yucca elephantipes was studied. The results showed that the terminal bud of Yucca elephantipes was planted in the culture medium MS+6-BA 2.0 mg/L+NNA 0.1 mg/L after being disinfected ,then the callus and adventitious bud were induced. The expansion rate was 2.2 after being subcultured in 1/2MS+6-BA 1.0 mg/L+NAA 0.1 mg/L. Then the subculture buds long than 3 cm were inoculated in rooting medium 1/2MS+IAA 0.3 mg/L, and the rooting rate reached 98.2% after 30 days. The survival rate of transplant was high than 95% after 20-30 clays adaptation.