CAJ | 학술논문

利用前人已报道的经典的KA系列、LC系列及MT系列简并引物扩增StreptomycesJK-1基因组DNA，共获得10条目标片段，所有序列的GC含量均在70％左右，具有链霉菌的高GC含量特性。其中片段1与S．coelicolorA3（2）的AL939117．1基因序列在50％可比对序列上有90％的相似性，片段2与S．ambofaciensATCC23877的Am238663．1基因序列在95％可比对序列上有8l％的相似性，片段10与S．avermitilisMA-4680的BA000030．3基因序列在93％可比对序列上有85％的相似性。其余7条序列与GeneBank数据库中的序列无相似性。分析结果表明，已获得了新的PKS基因的部分片段，这些片段可以作为下一步获得PKS基因全长的有效探针。
이용전인이보도적경전적KA계렬、LC계렬급MT계렬간병인물확증StreptomycesJK-1기인조DNA，공획득10조목표편단，소유서렬적GC함량균재70％좌우，구유련매균적고GC함량특성。기중편단1여S．coelicolorA3（2）적AL939117．1기인서렬재50％가비대서렬상유90％적상사성，편단2여S．ambofaciensATCC23877적Am238663．1기인서렬재95％가비대서렬상유8l％적상사성，편단10여S．avermitilisMA-4680적BA000030．3기인서렬재93％가비대서렬상유85％적상사성。기여7조서렬여GeneBank수거고중적서렬무상사성。분석결과표명，이획득료신적PKS기인적부분편단，저사편단가이작위하일보획득PKS기인전장적유효탐침。
In this study, KA- series ,LC -series and MT- series degenerate primers were used to amplify polyketide syn- thase genes (PKSs) from Streptomyces JK- 1 resistance to Magnaporthe oryzae. As a result, 10 bands were obtained, and the GC content of all sequences was about 70 percent. In all sequences, 50 percent of sequence 1 was match to the sequence of AL939117.1 gene of S. coelicolor A3(2), and showed 90% similarity; 95 percent of sequence 2 was match to the se- quence of Am238663.1 gene of S. ambofaciens ATCC 23877, and showed 81% similarity; and 93 percent of sequence 10 was match to the sequence of BA000030.3 gene of S. avermitilis MA -4680, and showed 85% similarity. The other se- quences have no any similarity with the sequences in GeneBank database. The result showed that new PKS gene fragments were obtained. These sequences can be used as gene probe to obtain overall length PKS genes of Streptomyces JK - 1.