CAJ | 학술논문

目的：研究高水平17β-雌二醇持续刺激去卵巢小鼠，对子宫组织DLL1基因表达的影响。方法：将16只小鼠随机平均分为正常对照组和去卵巢E2实验组（手术造模＋E2干预）；TRIzol一步法提取两组小鼠子宫组织总RNA，采用RT-PCR法扩增目的基因及内参基因的目的片段，紫外光下观察电泳结果并采用QuantityOne-4．6．2凝胶分析软件测定目标带光强度值。结果：两组小鼠子宫组织标本均出现目的基因DLL1与内参基因Gapdh的目标带，DLL1/Gapdh光强度的比值分别为0．33±0．12、0．78±0．11，两者差异有统计学意义（P＜0．05），并且去卵巢E2实验组明显高于正常对照组。结论：高剂量E2的持续干预可显著提高去卵巢小鼠子宫组织DLL1基因的表达水平，可能是雌激素依赖性子宫组织细胞不正常增殖的病理基础。
목적：연구고수평17β-자이순지속자격거란소소서，대자궁조직DLL1기인표체적영향。방법：장16지소서수궤평균분위정상대조조화거란소E2실험조（수술조모＋E2간예）；TRIzol일보법제취량조소서자궁조직총RNA，채용RT-PCR법확증목적기인급내삼기인적목적편단，자외광하관찰전영결과병채용QuantityOne-4．6．2응효분석연건측정목표대광강도치。결과：량조소서자궁조직표본균출현목적기인DLL1여내삼기인Gapdh적목표대，DLL1/Gapdh광강도적비치분별위0．33±0．12、0．78±0．11，량자차이유통계학의의（P＜0．05），병차거란소E2실험조명현고우정상대조조。결론：고제량E2적지속간예가현저제고거란소소서자궁조직DLL1기인적표체수평，가능시자격소의뢰성자궁조직세포불정상증식적병리기출。
Objective:To examine the effects of constant high-dose 17β-estrogen stimulation on the Delta-like protein 1(DLL1) level in the uterine tissue of ovariectomized mice.Methods:Sixteen mice were randomly divided into normal control group and ovariectomized E2 experimental group (Model +E2 intervention).TRIzol reagent was used to extract the total RNA from the uterine tissues,and reverse transcription polymerase chain reaction(RT-PCR) was used to amplify the target sequences and internal control gene that were observed under UV light measured for the value of Adj .Vol with QuantityOne-4.6.2.Results:The target bands of gene DLL1 and Gapdh were detected in all samples,and the DLL1/Gapdh Adj.Vol.value of the two groups were 0.33 ±0.12,0.78 ±0.11,respectively.The two groups were statistically different(P <0.05),and the expression level was significantly higher in E2 group than the normal control group.Conclusion:Lasting high-dose estrogen stimulation could increase the gene DLL1expression in the uterine tissue of mice,which may explain the pathomechanism for abnormal growing of uterine tissue resulted from estrogen dependence .