CAJ | 학술논문

目的：观察芝麻素（Ses）对自发性高血压大鼠（SHR）的降压作用，并探讨其可能机制。方法：SHR 35只，随机分为SHR模型组、Ses160、80、40 mg/（kg· d）、卡托普利（Cap）30 mg/（kg· d），另设WKY正常对照组，于给药16周后，颈总动脉插管测收缩压（SBP）、舒张压（DBP）及平均血压（MBP），Griess Reagent法测主动脉中一氧化氮（NO）含量，化学比色法测主动脉中过氧化氢（H2O2）含量，RT-PCR观察主动脉内皮型一氧化氮合酶（eNOS）、p22phox及p47phox mRNA表达。结果：与SHR组相比，Ses160、80 mg/kg组SBP、DBP、MAP均显著降低（P＜0．01，P＜0．05），主动脉中NO含量显著提高（P＜0．01），eNOS mRNA表达显著增加（P＜0．01，P＜0．05），p22phox、p47phox mRNA表达显著减少（P＜0．01，P＜0．05）。 Ses各剂量组H2O2含量显著降低（P＜0．01）。结论：芝麻素通过上调SHR主动脉中eNOS mRNA的表达，使内皮细胞合成和释放NO增多；下调主动脉NAD-PH氧化酶p22phox和p47phox mRNA的表达，使NO活性恢复，发挥降血压的作用。
목적：관찰지마소（Ses）대자발성고혈압대서（SHR）적강압작용，병탐토기가능궤제。방법：SHR 35지，수궤분위SHR모형조、Ses160、80、40 mg/（kg· d）、잡탁보리（Cap）30 mg/（kg· d），령설WKY정상대조조，우급약16주후，경총동맥삽관측수축압（SBP）、서장압（DBP）급평균혈압（MBP），Griess Reagent법측주동맥중일양화담（NO）함량，화학비색법측주동맥중과양화경（H2O2）함량，RT-PCR관찰주동맥내피형일양화담합매（eNOS）、p22phox급p47phox mRNA표체。결과：여SHR조상비，Ses160、80 mg/kg조SBP、DBP、MAP균현저강저（P＜0．01，P＜0．05），주동맥중NO함량현저제고（P＜0．01），eNOS mRNA표체현저증가（P＜0．01，P＜0．05），p22phox、p47phox mRNA표체현저감소（P＜0．01，P＜0．05）。 Ses각제량조H2O2함량현저강저（P＜0．01）。결론：지마소통과상조SHR주동맥중eNOS mRNA적표체，사내피세포합성화석방NO증다；하조주동맥NAD-PH양화매p22phox화p47phox mRNA적표체，사NO활성회복，발휘강혈압적작용。
Objective:To observe the anti-hypertensive effects of sesamin ( Ses) and its potential mechanism in the spontaneously hypertensive rats (SHR).Methods:Thirty-five SHRs were randomized into groups of SHR model,administration of sesamin in dose of 160,80 and 40 mg/(kg· d) as well as captopril(Cap)in dose of 30 mg/(kg· d).Another 7 Wistar-Kyoto (WKY) rats were added as negative controls.All rats were undergone measurement of the systolic blood pressure( SBP) ,diastolic blood pressure( DBP) and mean blood pressure( MBP) via inserting a cannula into the carotids,and deter-mination of the aortic nitric oxide (NO) and hydrogen peroxide (H2O2) levels with Griess reagent.Reverse transcription polymerase chain reaction(RT-PCR) was performed to determine the expression of aortic eNOS,p22phox and p47phox mRNA.Results:The SBP,DBP and MAP were significantly decreased in groups treated with sesamin in dose of 160 and 80 mg/kg(P<0.01,P<0.05),whereas the aortic NO level was markedly elevated (P<0.01) and eNOS mRNA expression was remarkably up-regulated(P<0.01,P<0.05),and p22phox and p47phox mRNA were significantly down-regulated(P<0.01, P<0.05),as compared with those in the SHR group.In addition,aortic H2O2 levels were reduced in groups treated with diverse dose of sesamin(P<0.01).Conclusion:Sesamin may function in anti-hypertension for SHR through up-regulating the level of eNOS mRNA to facilitate increased synthesis and release of NO in the endothelial cells,while down-regulating the subunits of p22phox and p47phox mRNA expression in aortas to enhance the NO activity.