CAJ | 학술논문

目的：观察电针对脑缺血再灌注损伤后大鼠额叶皮质和海马CA1区Bcl-2、Bax表达的影响。方法：将24只SD大鼠随机分为假手术组、模型组和电针组。采用大脑中动脉线栓法制备脑缺血再灌注模型。取百会和大椎穴给予电针刺激，频率2 Hz，持续30 min，每天1次，连续7 d。免疫组织化学方法观察额叶皮质和海马CA1区Bcl-2、Bax的表达。结果：假手术组大鼠神经功能缺损评分均为0分，模型组神经功能缺损评分高于电针治疗组，两组间有显著性差异（ P＜0．01）。模型组Bcl-2在额叶皮质和海马CA1区的表达与假手术组比较无明显变化（ P＞0．05），电针组Bcl2-的表达明显高于模型组（ P＜0．01）。模型组Bax在额叶皮质和海马CA 1区的表达明显高于假手术组（P＜0．01），电针组Bax的表达显著低于模型组（P＜0．01或P＜0．05）。结论：电针治疗可改善脑缺血再灌注大鼠神经功能，可能与其促进Bcl-2、抑制Bax的表达有关。
목적：관찰전침대뇌결혈재관주손상후대서액협피질화해마CA1구Bcl-2、Bax표체적영향。방법：장24지SD대서수궤분위가수술조、모형조화전침조。채용대뇌중동맥선전법제비뇌결혈재관주모형。취백회화대추혈급여전침자격，빈솔2 Hz，지속30 min，매천1차，련속7 d。면역조직화학방법관찰액협피질화해마CA1구Bcl-2、Bax적표체。결과：가수술조대서신경공능결손평분균위0분，모형조신경공능결손평분고우전침치료조，량조간유현저성차이（ P＜0．01）。모형조Bcl-2재액협피질화해마CA1구적표체여가수술조비교무명현변화（ P＞0．05），전침조Bcl2-적표체명현고우모형조（ P＜0．01）。모형조Bax재액협피질화해마CA 1구적표체명현고우가수술조（P＜0．01），전침조Bax적표체현저저우모형조（P＜0．01혹P＜0．05）。결론：전침치료가개선뇌결혈재관주대서신경공능，가능여기촉진Bcl-2、억제Bax적표체유관。
Objective:To observe the effects of electroacupuncture(EA) on Bcl-2and Bax expression in the frontal cortex and hippocampus CA 1 area of rats after focal cerebral ischemia-reperfusion.Methods:Twenty-four Sprague-Dawley( SD) rats were randomized into groups of sham operation ,model and EA.The models of focal cerebral ischemiar-eperfusion were induced by intraluminal middle cerebral artery occlusion ( MCAO) with a nylon monofilament suture,and EA was performed at the acupoints of Baihui and Dazhui for the EA group in consecutive 7 days by lasting for 30 min, once a day.Immunohis-tochemical technique was used to detect the expression of Bcl-2 and Bax in frontal cortex and hippocampus CA1 area.Results:The neurologic impairment score was 0 for the sham operation group,and the model group scored significantly higher than the EA group with statistical difference(P<0.01).Bcl-2 expression remained no significant change in frontal cortex and hippocampus CA1 area between the sham operation group and model group(P>0.05),yet was higher in EA group(P<0.01).Bax expression was significantly increased in the frontal cortex and hippocampus CA1 area in the model group as com-pared with the sham operation group(P<0.01),but remarkably lower in the EA group than that in the model group(P<0.01,P<0.05).Conclusion:Electroacupuncture intervention has improved the nerve function of rats after focal cerebral ischemia-reperfusion,which process may be associated with en-hanced Bcl-2 level but suppressed Bax expression.