CAJ | 학술논문

目的：观察雷公藤多甙对单侧输尿管梗阻（UUO）大鼠模型中结缔组织生长因子（CTGF）表达的影响。方法输尿管梗阻大鼠随机分为模型组、贝那普利组、雷公藤多甙组、假手术组，雷公藤多甙与贝那普利组以药物10 mg· kg-1· d-1溶于5 mL生理盐水灌胃，模型及假手术组以等体积生理盐水灌胃，术后14 d分别处死各组8只大鼠。采用免疫组化（SABC法）检测各组大鼠肾小管间质 CTGF的表达水平。结果模型组、贝那普利组和雷公藤多甙组大鼠肾小管间质CTGF表达均高于假手术组，差异有统计学意义（P ＜0．01）。雷公藤多甙组大鼠肾小管间质的CTGF表达显著低于模型组（P ＜0．01），亦低于贝那普利组（P ＜0．05）。结论在肾小管间质纤维化中雷公藤多甙起着重要的治疗作用，可能与抑制TGF-β相关。雷公藤多甙可以下调 CTGF表达，减轻肾小管间质损伤，可作为梗阻性肾病的辅助治疗用药。
목적：관찰뢰공등다대대단측수뇨관경조（UUO）대서모형중결체조직생장인자（CTGF）표체적영향。방법수뇨관경조대서수궤분위모형조、패나보리조、뢰공등다대조、가수술조，뢰공등다대여패나보리조이약물10 mg· kg-1· d-1용우5 mL생리염수관위，모형급가수술조이등체적생리염수관위，술후14 d분별처사각조8지대서。채용면역조화（SABC법）검측각조대서신소관간질 CTGF적표체수평。결과모형조、패나보리조화뢰공등다대조대서신소관간질CTGF표체균고우가수술조，차이유통계학의의（P ＜0．01）。뢰공등다대조대서신소관간질적CTGF표체현저저우모형조（P ＜0．01），역저우패나보리조（P ＜0．05）。결론재신소관간질섬유화중뢰공등다대기착중요적치료작용，가능여억제TGF-β상관。뢰공등다대가이하조 CTGF표체，감경신소관간질손상，가작위경조성신병적보조치료용약。
Objective To observe the effect of tripterygium wilfordii on expression of tubulointerstitial con-necti-ve tissue growth factor (CTGF)in unilateral ureter obstruction (UUO)rats.Methods 32 UUO rats were divided into four groups:sham surgery group,model group, Benazepril group, Tripterygium wilfordii group.Rats were sacrificed after 14 days .The expression of CTGF was detected by immunohisto-chemi-stry.Results Contrast the sham group,the level of CTGF expression is obviously higher than other three groups (P <0.01).Ripterygium wilfordii group is lower than model group (P <0.01),the CTGF of ripterygium wilfordii.group is lower than Benazepril group (P < 0.05 ).Conclusion Tripterygium wilfordii can degrade the expression of UUO CTGF level and reduce tubulointerstitial inj ury. It is a adj unct therapy on obstruction kidney disease.