CAJ | 학술논문

本试验通过PCR方法对17株广西猪源猪链球菌2型（SS2）荚膜多糖（CPS）基因cps2J片段进行了扩增、克隆测序，同源性分析结果表明，17株广西SS2的cps2J基因核苷酸和氨基酸的同源性分别在91.9%～99.8%和89.1%～99.7%；菌株可以分为I群和II群，包括5株发病猪源菌株的16株菌均分在I群，II群只有GXWX193株。I群菌株的CPS基因核苷酸和氨基酸的同源性分别在96.8%～99.8%和94.6%～99.7%，与参考菌（荷兰Netherland、猪源菌株江苏ZYS8和四川SC17、人源菌株江苏98015）的核苷酸和氨基酸的同源性分别在96.4%～99.9%和94.1%～100%。II群菌株与参考菌株的核苷酸和氨基酸同源性分别在91.3%～92.4%和89.1%～91.0%。17株广西猪源SS2菌株的cps2J基因序列变异程度小。
본시험통과PCR방법대17주엄서저원저련구균2형（SS2）협막다당（CPS）기인cps2J편단진행료확증、극륭측서，동원성분석결과표명，17주엄서SS2적cps2J기인핵감산화안기산적동원성분별재91.9%～99.8%화89.1%～99.7%；균주가이분위I군화II군，포괄5주발병저원균주적16주균균분재I군，II군지유GXWX193주。I군균주적CPS기인핵감산화안기산적동원성분별재96.8%～99.8%화94.6%～99.7%，여삼고균（하란Netherland、저원균주강소ZYS8화사천SC17、인원균주강소98015）적핵감산화안기산적동원성분별재96.4%～99.9%화94.1%～100%。II군균주여삼고균주적핵감산화안기산동원성분별재91.3%～92.4%화89.1%～91.0%。17주엄서저원SS2균주적cps2J기인서렬변이정도소。
Amplified and cloned by PCR, then sequenced the virulence-associated factor cps2J gene of 17 strains of type 2 Streptococcus suis isolated from Guangxi. The homologies of nucleo-tide and predicted amino acids of 17 Guangxi SS2 are 91.9%~99.8% and 89.1%~99.7% respectively.17 SS2 strains could be divided into two groups as I (11 strains isolated from healthy pigs and 5 strains from diseased pigs) and II (1 strain isolated from healthy pigs). The homologies of group I were very high (96.8%~99.8% nt and 94.6%~99.7% aa). Compared to reference strains(Netherland/ZYS8/SC17/98015), homologies of group I were 96.4%~99.9%nt and 94.1%~100%aa , 91.3%~92.4%nt and 89.1%~91.0%aa in group II. There is a low degree of variation in cps2J gene in 17 SS2 isolated from pigs in Guangxi ,China.