CAJ | 학술논문

本研究通过荧光定量PCR方法检测了TLR15、LY86和TRIM39 mRNA在不同日龄鸡法氏囊中的变化。结果显示，10日龄时，TLR15 mRNA的相对表达量显著高于18胚龄时的，10日龄和30日龄时的表达量基本相同；出壳后，LY86 mRNA的表达水平呈下降趋势，30日龄时的表达量极显著低于18胚龄时的；而TRIM39 mRNA在出壳后表达量逐渐增加，30日龄时的表达量极显著高于18胚龄时的。本研究结果表明，TLR15、LY86和TRIM39在鸡法氏囊发育过程中发挥着重要作用。
본연구통과형광정량PCR방법검측료TLR15、LY86화TRIM39 mRNA재불동일령계법씨낭중적변화。결과현시，10일령시，TLR15 mRNA적상대표체량현저고우18배령시적，10일령화30일령시적표체량기본상동；출각후，LY86 mRNA적표체수평정하강추세，30일령시적표체량겁현저저우18배령시적；이TRIM39 mRNA재출각후표체량축점증가，30일령시적표체량겁현저고우18배령시적。본연구결과표명，TLR15、LY86화TRIM39재계법씨낭발육과정중발휘착중요작용。
The expression levels of Toll-like receptor 15 (TLR15), Lymphocyte antigen 86 (LY86), Tripartite motif 39 (TRIM39) mRNA in bursa of Fabriciuss of different days-old chickens was detect-ed by real-time quantitative PCR. The results showed that the expression level of TLR15 mRNA in chicken bursa of Fabricius at 10 day-old was significantly higher than the level at 18 embryo-old, while the expression level of TLR15 mRNA was almost the same between 10 and 30 day-old. The expres-sion level of LY86 mRNA decreased after post-hatched, and the level of LY86 mRNA at 30 day-old was significantly lower than the level at 18 embryo-old. The expression level of TRIM39 mRNA increased after post-hatched, and the level of TRIM39 mRNA at 30 day-old significantly higher than the level at 18 embryo-old. The findings suggested TLR15, LY86 and TRIM39 have vital roles during the course of the development in bursa of Fabricius.