CAJ | 학술논문

腐蹄病是指侵害指（趾）间皮肤及皮肤更深层软组织的急性或亚急性炎症。患病皮肤常坏死、裂开，炎症从指（趾）间皮肤蔓延到蹄冠、系部和球节，病肢明显跛行，并伴有全身性症候。腐蹄病是奶牛蹄病中危害性极为严重的一类疾病，病畜食欲减退、泌乳量下降、繁殖能力降低，病情严重者将被迫淘汰，严重影响奶牛业的发展，造成严重的经济损失。鉴于该病对奶牛业的严重危害，奶牛出现蹄病时的及时、准确临床诊断，以及与其他蹄病的鉴别诊断，对于奶牛蹄病的临床治疗和预防具有重要的实际指导意义。本试验根据节瘤拟杆菌纤毛蛋白基因序列的特异性，设计1对引物BPP并建立了PCR检测方法。试验结果表明，引物BPP具有相对较高的特异性和敏感性，其敏感性达到了152.5 pg，且引物FLP具有很好的特异性，能够准确诊断出节瘤拟杆菌的存在。
부제병시지침해지（지）간피부급피부경심층연조직적급성혹아급성염증。환병피부상배사、렬개，염증종지（지）간피부만연도제관、계부화구절，병지명현파행，병반유전신성증후。부제병시내우제병중위해성겁위엄중적일류질병，병축식욕감퇴、비유량하강、번식능력강저，병정엄중자장피박도태，엄중영향내우업적발전，조성엄중적경제손실。감우해병대내우업적엄중위해，내우출현제병시적급시、준학림상진단，이급여기타제병적감별진단，대우내우제병적림상치료화예방구유중요적실제지도의의。본시험근거절류의간균섬모단백기인서렬적특이성，설계1대인물BPP병건립료PCR검측방법。시험결과표명，인물BPP구유상대교고적특이성화민감성，기민감성체도료152.5 pg，차인물FLP구유흔호적특이성，능구준학진단출절류의간균적존재。
Footrot is also called interdigital Phlegmon, which is a kind of acute or subacute in-flammation that affected interdigital skin even deeper soft tissue. The disease characterized by the separation of keratinous hoof from underlying epidermal tissue, inflammation often spread from interdigital skin to coronet and fetlock. The prognosis of footrot is lameness, even could not stand and exhibit general systemic symptom. Footrot is a kind of serious disease in cow hoof dis-eases, which could decreased appetite, milk secretion and also reduced the productivity of cattle farm, causing a severe economic loss to dairy farm and interfer the development of cattle indus-try. Therefore, it is important to study a rapid diagnosis method of Footrot, which may have the practical guidance significance in footrot final diagnosis, clinical cure and prophylaxis. Accord-ing to Bacteroides nodosus gene sequence, we designed primers BPP and set up PCR method. Experimen-tal result indicated that primers BPP possess higher specificity and sensitivity. The sensitivity has achieved 152.5 pg, and BPP possess commendable specificity, which can provide a definite diag-nosis for the presence of Bacteroides nodosus.