中外食品工业(下半月)
中外食品工業(下半月)
중외식품공업(하반월)
Sino-foreign Food Industry
2014年
3期
33-34
,共2页
植物油%苯并(а)芘%高效液相色谱
植物油%苯併(а)芘%高效液相色譜
식물유%분병(а)비%고효액상색보
vegetable oil%benzopyrene High Performance%Liquid Chromatography
采用C18(250 mm×4.6mm×5μm)反相色谱柱,在流动相为乙腈:水(88∶12,v/v),流速为1.0 ml/min,激发波长384 nm,发射波长406 nm,柱温35℃,进样量10μl 的条件下,建立高效液相色谱法快速测定植物油中苯并芘残留量的方法。结果:本方法的检出限为0.5μg/kg,加标回收率95%~103%,相对标准偏差RSD≤2.7%。该法具有样品预处理简便,试剂消耗少,分析时间短、精密度高等优点,适用于植物油中苯并芘残留量的高效测定。
採用C18(250 mm×4.6mm×5μm)反相色譜柱,在流動相為乙腈:水(88∶12,v/v),流速為1.0 ml/min,激髮波長384 nm,髮射波長406 nm,柱溫35℃,進樣量10μl 的條件下,建立高效液相色譜法快速測定植物油中苯併芘殘留量的方法。結果:本方法的檢齣限為0.5μg/kg,加標迴收率95%~103%,相對標準偏差RSD≤2.7%。該法具有樣品預處理簡便,試劑消耗少,分析時間短、精密度高等優點,適用于植物油中苯併芘殘留量的高效測定。
채용C18(250 mm×4.6mm×5μm)반상색보주,재류동상위을정:수(88∶12,v/v),류속위1.0 ml/min,격발파장384 nm,발사파장406 nm,주온35℃,진양량10μl 적조건하,건립고효액상색보법쾌속측정식물유중분병비잔류량적방법。결과:본방법적검출한위0.5μg/kg,가표회수솔95%~103%,상대표준편차RSD≤2.7%。해법구유양품예처리간편,시제소모소,분석시간단、정밀도고등우점,괄용우식물유중분병비잔류량적고효측정。
A method for determination of benzopyrene in vegetable oil by High Performance Liquid Chromatography was established. Using C18 (250mm×4.6mm×5μm) reversed-phase column, with acetonitrile and H2O (88∶12,v/v) as mobile phase,flow rate was 1.0 ml/min, and excitation wavelength was 384nm and emission wavelength was 406 nm, and column temperature was 35℃, and injection volume was 10μl. Results:The detection limit was 0.5μg/kg, and the recovery rate arange from 95%to 103%, and relative standard deviation RSD was≤2.7%. This method is simple preparation, reagent consumption, short analysis time, precision advantages for vegetable oils and efficient determination of benzopyrene residues.