明胶科学与技术
明膠科學與技術
명효과학여기술
THE SCIENCE AND TECHNOLOGY OF GELATIN
2012年
3期
113-126
,共14页
刘闯%唐晋%肖文君%郭玉龙%刘善堂%彭必先
劉闖%唐晉%肖文君%郭玉龍%劉善堂%彭必先
류틈%당진%초문군%곽옥룡%류선당%팽필선
十二烷基硫酸钠聚丙烯酰胺凝胶电泳%棒状胶原水解物(1-100kD)
十二烷基硫痠鈉聚丙烯酰胺凝膠電泳%棒狀膠原水解物(1-100kD)
십이완기류산납취병희선알응효전영%봉상효원수해물(1-100kD)
SDS - PAGE%rod - like hydealyzed conagens ( 1 - 100kD)
采用凝胶板配方和电泳条件优化后的SDS—PAGE技术对酸法明胶水解物、碱法明胶水解物、胃蛋白酶法明胶水解物、胃蛋白酶法猪皮胶原水解物、明胶以及猪皮胶原蛋白透析物等进行表征,获得了将分布于一块凝胶板上的1~100kD的棒状胶原蛋白各组分的色谱图。胃蛋白酶法猪皮胶原蛋白水解物经电泳分离后的色谱图中,各谱带清晰明显,分子量均一,且均匀分布于1~100kD不等,这对棒状胶原蛋白分子的研究具有重要价值。
採用凝膠闆配方和電泳條件優化後的SDS—PAGE技術對痠法明膠水解物、堿法明膠水解物、胃蛋白酶法明膠水解物、胃蛋白酶法豬皮膠原水解物、明膠以及豬皮膠原蛋白透析物等進行錶徵,穫得瞭將分佈于一塊凝膠闆上的1~100kD的棒狀膠原蛋白各組分的色譜圖。胃蛋白酶法豬皮膠原蛋白水解物經電泳分離後的色譜圖中,各譜帶清晰明顯,分子量均一,且均勻分佈于1~100kD不等,這對棒狀膠原蛋白分子的研究具有重要價值。
채용응효판배방화전영조건우화후적SDS—PAGE기술대산법명효수해물、감법명효수해물、위단백매법명효수해물、위단백매법저피효원수해물、명효이급저피효원단백투석물등진행표정,획득료장분포우일괴응효판상적1~100kD적봉상효원단백각조분적색보도。위단백매법저피효원단백수해물경전영분리후적색보도중,각보대청석명현,분자량균일,차균균분포우1~100kD불등,저대봉상효원단백분자적연구구유중요개치。
An attempt was made to use CB - peptides as new makers and to optimise the gel formulas and electrophoresis conditions for SDS -polyaerylamide gel electrophoresis to char~erise the acid -hydrolyzed gelatin, .alkali -hydmlyzed gelatin, pepsinhydrolyzed gelatin as well as pepsin- hydrolyzed pig skin collagen. The different components of rod - like collagen hydrolyses with molecular weight possibly ranging from I to 100 kDalton can be separated, displayed clearly in their electrophoresis scanned chromatograms and quantitatively characterised successfizlly. In a typical electrophoresls chroraatogram d pepsin hydrolyzed pig skin collagen, all the bands were dearly localised and their molecular weights orderly were shon to be dislributed between 1 and 100 kD after calibrating. All these are of important values foz the test of collagen hydrolyses.