CAJ | 학술논문

本文通过提取、分离、鉴定连翘（Forsythia suspensa）中酪氨酸酶抑制成分,并检测其酪氨酸酶抑制活性.采用的方法是利用蘑菇酪氨酸酶对连翘进行抑制活性筛选,并通过硅胶柱色谱法分离、鉴定酪氨酸酶抑制成分.结果发现,对连翘的乙醇提取物及其二氯甲烷、正丁醇、水萃取物进行酪氨酸酶抑制活性检测结果均有浓度依赖性的抑制活性,尤其是连翘的二氯甲烷萃取物显示最强的活性,并通过硅胶柱色谱法分离得到有效成分,利用核磁共振、质谱等分析手段有效成分被鉴定为白桦脂酸,其酪氨酸酶的半数抑制率IC50值为138.5±1.57μmol/L.结论：连翘具有较强的酪氨酸酶抑制活性,为连翘的有效利用提供实验依据.
본문통과제취、분리、감정련교（Forsythia suspensa）중락안산매억제성분,병검측기락안산매억제활성.채용적방법시이용마고락안산매대련교진행억제활성사선,병통과규효주색보법분리、감정락안산매억제성분.결과발현,대련교적을순제취물급기이록갑완、정정순、수췌취물진행락안산매억제활성검측결과균유농도의뢰성적억제활성,우기시련교적이록갑완췌취물현시최강적활성,병통과규효주색보법분리득도유효성분,이용핵자공진、질보등분석수단유효성분피감정위백화지산,기락안산매적반수억제솔IC50치위138.5±1.57μmol/L.결론：련교구유교강적락안산매억제활성,위련교적유효이용제공실험의거.
Objective: Extraction, Isolation and Identification of tyrosinase inhibitory active ;i constituent From Forsythia suspensa. Method: The fruits of F. suspensa were screened by mushroom tyrosin'ase, and the tyrosinase inhibitory active constituent was isolated via silica gel chromatography and identified by NMR and Mass spectra. Result: The ethanol extract, and fractions of CH2C12, BuOH and H2O from F. suspensa showed the tyrosianse inhibitory activity in a dose-dependent manner. In particular, the CH2 C12 fraction appeared the most activity. The tyrosinase inhibitory active constituent was isolated by silica gel chromatography and identified as betulinic acid, showing the ICs0 value of 138.5 ±1.57μmol/L. Conclusion: F. suspensa showed strong tyrosinase inhibitory activity, and it was provided for the effective use of F. suspensa.