CAJ | 학술논문

以花叶艳山姜（Alpinia zerumbet cv．vaniegata）的茎尖作为外植体，研究了花叶艳山姜的离体快繁技术。结果表明：花叶艳山姜茎尖接种在MS＋BA3．0mg·L-1＋NAA0．2mg·L-1＋白糖30g·L-1的培养基上，30d后长出幼芽；丛生芽在MS＋6-BA2．0—2．5mg·L-1＋NAA0．2mg·L-1＋白糖30g·L-1的培养基上，增殖效果最佳，增殖倍数达4．0，芽生长正常，且色泽浓绿；芽苗在1／2MS＋NAA1．0～1．5mg·L-1＋白糖30g·L-1的培养基上，生根效果较好，根系粗壮，生根率为93．0％；组培苗移栽在V河沙：V珍珠岩：V表土：1：1：1的混合基质中，成活率可达70．1％。
이화협염산강（Alpinia zerumbet cv．vaniegata）적경첨작위외식체，연구료화협염산강적리체쾌번기술。결과표명：화협염산강경첨접충재MS＋BA3．0mg·L-1＋NAA0．2mg·L-1＋백당30g·L-1적배양기상，30d후장출유아；총생아재MS＋6-BA2．0—2．5mg·L-1＋NAA0．2mg·L-1＋백당30g·L-1적배양기상，증식효과최가，증식배수체4．0，아생장정상，차색택농록；아묘재1／2MS＋NAA1．0～1．5mg·L-1＋백당30g·L-1적배양기상，생근효과교호，근계조장，생근솔위93．0％；조배묘이재재V하사：V진주암：V표토：1：1：1적혼합기질중，성활솔가체70．1％。
Shoot tips were used as explants to mass propagate Alpinia zerumbet cv. vaniegata. The shoot tips were cultured on the MS medium at the presence of BA 3.0 mg · L-1 + NAA 0.2 mg · L- 1 + sugar 30 mg·L- 1 for 30 days and produced adventitious shoots continuously. These shoots were transferred onto the MS medium sup- plemented with 6 - BA 2.0--2.5 mg · L-1 + NAA 0.2 mg · L- 1 + sugar 30 mg · L-1 for further culture and were well proliferated with a proliferation coefficient of 4.0. The multiplied shoots grew normal with dark green color and were rooted well with stout roots on the 1/2MS medium supplemented with NAA 1.0--1.5 mg · L-1 with a rooting rate of 93.0%. The rooted plantlets of A. zerumbet cv. vaniegata were transplanted in the mixed substrate ( river sand: perlite: surface soil = 1 : 1 : 1 ), and survived upto 70.1%.