热带生物学报
熱帶生物學報
열대생물학보
JOURNAL OF SOUTH CHINA UNIVERSITY OF TROPICAL AGRICULTURE
2012年
3期
243-246
,共4页
八角茴香提取物%DPPH自由基%抗氧化活性
八角茴香提取物%DPPH自由基%抗氧化活性
팔각회향제취물%DPPH자유기%항양화활성
star anise extract%DPPH free radical%antioxidant activity
摘要:以蒸馏水、乙醇、丙酮、正丁醇为溶剂对八角茴香进行提取,采用DPPH法对各提取物进行自由基清除实验,同时,以挥发性油和乙酸乙酯提取物作为抗氧化剂,定量加入油脂中,通过对强化保存期间过氧化值(POV)的测定,比较它们不同的添加量对猪油抗氧化性能的影响。结果表明,同一质量浓度不同溶剂的提取物对DPPH自由基的清除能力作用强弱是:乙醇提取物〉正丁醇提取物〉丙酮提取物〉蒸馏水提取物;挥发性油抗氧化效果不明显,乙酸乙酯提取物具有很强的抗氧化活性,当乙酸乙酯提取物添加量达w=0.10%时,抗氧化效果优于w=0.02%的BHT,且添加量越高抗氧化效果越好。
摘要:以蒸餾水、乙醇、丙酮、正丁醇為溶劑對八角茴香進行提取,採用DPPH法對各提取物進行自由基清除實驗,同時,以揮髮性油和乙痠乙酯提取物作為抗氧化劑,定量加入油脂中,通過對彊化保存期間過氧化值(POV)的測定,比較它們不同的添加量對豬油抗氧化性能的影響。結果錶明,同一質量濃度不同溶劑的提取物對DPPH自由基的清除能力作用彊弱是:乙醇提取物〉正丁醇提取物〉丙酮提取物〉蒸餾水提取物;揮髮性油抗氧化效果不明顯,乙痠乙酯提取物具有很彊的抗氧化活性,噹乙痠乙酯提取物添加量達w=0.10%時,抗氧化效果優于w=0.02%的BHT,且添加量越高抗氧化效果越好。
적요:이증류수、을순、병동、정정순위용제대팔각회향진행제취,채용DPPH법대각제취물진행자유기청제실험,동시,이휘발성유화을산을지제취물작위항양화제,정량가입유지중,통과대강화보존기간과양화치(POV)적측정,비교타문불동적첨가량대저유항양화성능적영향。결과표명,동일질량농도불동용제적제취물대DPPH자유기적청제능력작용강약시:을순제취물〉정정순제취물〉병동제취물〉증류수제취물;휘발성유항양화효과불명현,을산을지제취물구유흔강적항양화활성,당을산을지제취물첨가량체w=0.10%시,항양화효과우우w=0.02%적BHT,차첨가량월고항양화효과월호。
Star anise ( lllicium verum Hook. f. ), an important source of natural anti-oxidants, was extracted with solvents of water, ethanol, actone and n- butanol, respectively. The extracts were determined in free radical scavenging activity by using 2,2-diphenyl-l-picrylhydrazyl (DPPH) assay. The volatile oil and the ethyl acetate extract from these extracts were used as antioxidants and added into the lard quantitatively, and the peroxide val- ue (POV) of the lard stored for a given time was measured to compare the influence of different rates of the vola- tile oil and the ethyl acetate extract on oxidation resistance of the lard. The results showed that the extracts varied in free radical scavenging activity which was the highest in the ethanol extract with the order of ethanol 〉 n- buta- nol 〉 actone 〉 water under the same concentration. The volatile oil was low in anti-oxidative activity while the ethyl acetate extract had very high anti-oxidative activity. The ethyl acetate extract was higher in oxidation resist- ance when added into the lard at a rate of 0.10% (w) than the butylated hydroxytoluene (BHT) at a rate of 0. 02% (w), and had higher anti-oxidative activity when added at a higher rate.