西华师范大学学报:自然科学版
西華師範大學學報:自然科學版
서화사범대학학보:자연과학판
Journal of China West Normal University:Natural Science Edition
2012年
4期
362-365
,共4页
鸡腿菇%担孢子%萌发%抗生素%抑菌
鷄腿菇%擔孢子%萌髮%抗生素%抑菌
계퇴고%담포자%맹발%항생소%억균
Coprinus comatus%basidiospores%germination%antibiotics%antibacterial
鸡腿菇成熟时子实体自溶成墨汁状液体,液体中含有多种微生物,这使得从中分离担孢子和通过单孢杂交进行良种选育变得困难.本文试验了四种抗生素在鸡腿菇担孢子萌发时对杂菌的抑制效果,结果表明头孢曲松钠浓度为125μg/mL时,抑菌效果最好;卡那霉素浓度为75μg/mL时,效果较好;硫酸庆大霉素浓度为20μg/mL时,也可选作从墨汁化液体中分离单孢子;链霉素表现为对杂菌和鸡腿菇担孢子的同步抑制,不适宜用来分离鸡腿菇担孢子.
鷄腿菇成熟時子實體自溶成墨汁狀液體,液體中含有多種微生物,這使得從中分離擔孢子和通過單孢雜交進行良種選育變得睏難.本文試驗瞭四種抗生素在鷄腿菇擔孢子萌髮時對雜菌的抑製效果,結果錶明頭孢麯鬆鈉濃度為125μg/mL時,抑菌效果最好;卡那黴素濃度為75μg/mL時,效果較好;硫痠慶大黴素濃度為20μg/mL時,也可選作從墨汁化液體中分離單孢子;鏈黴素錶現為對雜菌和鷄腿菇擔孢子的同步抑製,不適宜用來分離鷄腿菇擔孢子.
계퇴고성숙시자실체자용성묵즙상액체,액체중함유다충미생물,저사득종중분리담포자화통과단포잡교진행량충선육변득곤난.본문시험료사충항생소재계퇴고담포자맹발시대잡균적억제효과,결과표명두포곡송납농도위125μg/mL시,억균효과최호;잡나매소농도위75μg/mL시,효과교호;류산경대매소농도위20μg/mL시,야가선작종묵즙화액체중분리단포자;련매소표현위대잡균화계퇴고담포자적동보억제,불괄의용래분리계퇴고담포자.
The fruit bodies of shaggy mane will autolyse into ink-similar liquid when mature with many kinds of microorganisms. This makes it difficult of separating basidiospores and hybridization breeding by single spore hybridization. Four antibiotics are used to test the microbiostatic effects on basidiospore germination in Coprinus comatus. The results show that rocephin is the best when the concentration is 125μg/mL, kanamycin is better when the concentration is 75μg/ml, and gentamycin can also be used when its concentration is 20μg/mL, but streptomycin is not suitable for he separation of basidiospores 15ecause of its synchronous suppression for basidiospores germination and micro propagation.