CAJ | 학술논문

研究了酸性条件下染色剂苋菜红-蛋白质体系的共振瑞利散射光谱。在pH=3．80的Clark-Lubs缓冲介质中，苋菜红与蛋白质通过分子间作用力形成复合物，使最大波长为523nm的共振光散射光谱得到加强，这种增强的共振光散射用于鸡蛋清试样中总蛋白的测定，其线性响应范围为1．5～5．0mg／L，检测限为209μg／L。该方法的稳定性及选择性良好，适用于微量蛋白质分析。
연구료산성조건하염색제현채홍-단백질체계적공진서리산사광보。재pH=3．80적Clark-Lubs완충개질중，현채홍여단백질통과분자간작용력형성복합물，사최대파장위523nm적공진광산사광보득도가강，저충증강적공진광산사용우계단청시양중총단백적측정，기선성향응범위위1．5～5．0mg／L，검측한위209μg／L。해방법적은정성급선택성량호，괄용우미량단백질분석。
Resonance light scattering (RLS) spectrum of Amaranth -Protein system was studied in this paper. In Clark-Lubs buffer medium of pH 3.80, the Amaranth-Protein composites formed via intermolecular forces, which enhancing the resonance light scattering at maximum wavelength of about 523 nm. Based on the enhancement of RLS, a novel method for the determination of proteins was developed. The line arrange for the determination of protein is 1.5 to 5.0 mg/L, limit of detection is 209μg/L. The method is stability and selectivity, which can be used to determine trace protein.