CAJ | 학술논문

多杀菌素是新型生物源农药，系刺糖多孢菌的次级代谢产物。在单因子实验基础上，采用Plackett—Burman实验设计方法，优化了刺糖多孢菌培养条件。结果筛选出初始pH值、培养温度、摇瓶转速、摇瓶装液量4个因子，为本实验奈件下影响刺糖多孢菌生物量的主效因子，4因子贡献率总和达90．8％，建立了回归模型Y=7．73-0．63x1＋0．42x5-0．20x7＋0．20x8，决定系数R^2=0．9767，调整决定系数R^2=0．9359，方差分析表明模型显著，生物量最大预测值可达9．18g／L。
다살균소시신형생물원농약，계자당다포균적차급대사산물。재단인자실험기출상，채용Plackett—Burman실험설계방법，우화료자당다포균배양조건。결과사선출초시pH치、배양온도、요병전속、요병장액량4개인자，위본실험내건하영향자당다포균생물량적주효인자，4인자공헌솔총화체90．8％，건립료회귀모형Y=7．73-0．63x1＋0．42x5-0．20x7＋0．20x8，결정계수R^2=0．9767，조정결정계수R^2=0．9359，방차분석표명모형현저，생물량최대예측치가체9．18g／L。
Spinosad is a novel biological pesticide, which is secondary metabolites of Saccharopolyspora spinosa. On the basis of single factor experiments, optimization of culture conditions of Saccharopolyspora spinosa was studied by Plackett-Burman design.- Four main effect factors were screened out, including initial pH value, culture temperature, shaking speed, liquid volume, and their contribution to the model amounts to 90. 8%. Tile model was established, Y= 7. 73-0. 63x1 +0. 42x5 -0. 20x7 +0. 20x8, which was significant model by analysis of variance. The model's determination coefficient R-Squared was 0. 9767 and adjusted determination coefficient R-Squared was 0. 9359. According to the model, maximum predictive value of Saccharopolyspora spinosa biomass is 9. 18 g/L.