CAJ | 학술논문

以固相微萃取（SPME）顶空进样，采用GC—MS法测定两份海南沉香木精油A和B中的挥发成分，此精油是由中试规模的低温-动态-微波技术提取分离而得的．从相应精油中分别鉴定出58和68个成分，其中相同成分为41个．二者呈香成分的主要结构为倍半萜类，其中十氢萘型倍半萜相对含量最高，分别为42．35％和41．85％．因同一植物中生物合成的基础物质和途径往往相同，使其次生代谢产物的化学结构具有相似性，故推测二精油中含量最高成分的结构为十氢萘型倍半萜：乙酸-1，2，3，4，4a，7，8，8a-八氢-2，4a，5，8a-四甲基-1-萘酯（26．45％and19．21％）．二精油中含量较高的6个呈香成分均相同，表其香气特征相同；二者的品质差异：A中独有的保留时间较短的十氢化萘系列成分5个，表其头香特色不同于B；B中独有的保留时间较长的呈香成分14个，表其持香性较好．通过比较4种提取方法所获沉香精油的香气成分，表明SPME与GC—MS法是评价沉香精油品质的有效方法．
이고상미췌취（SPME）정공진양，채용GC—MS법측정량빈해남침향목정유A화B중적휘발성분，차정유시유중시규모적저온-동태-미파기술제취분리이득적．종상응정유중분별감정출58화68개성분，기중상동성분위41개．이자정향성분적주요결구위배반첩류，기중십경내형배반첩상대함량최고，분별위42．35％화41．85％．인동일식물중생물합성적기출물질화도경왕왕상동，사기차생대사산물적화학결구구유상사성，고추측이정유중함량최고성분적결구위십경내형배반첩：을산-1，2，3，4，4a，7，8，8a-팔경-2，4a，5，8a-사갑기-1-내지（26．45％and19．21％）．이정유중함량교고적6개정향성분균상동，표기향기특정상동；이자적품질차이：A중독유적보류시간교단적십경화내계렬성분5개，표기두향특색불동우B；B중독유적보류시간교장적정향성분14개，표기지향성교호．통과비교4충제취방법소획침향정유적향기성분，표명SPME여GC—MS법시평개침향정유품질적유효방법．
In this paper, the essential oils from Agarwood A and B collected in Hainan, China, were extracted by low temperature-dynamic microwave method. And the SPME-GC-MS technique was performed to analyze the volatile constituents in essential oil. 58 and 68 volatile components were identified from A and B, respectively, in which 41 compositions are the same, and the main volatile constituents in two essential oil were sesquiterpene in which the composition of the highest relative content was the decalin type ones accounting for 42.35% and 41.85%, respectively. Based on the biosynthetic nature that biosynthesis in the same plants tends to have the same origin and the way of synthesis, its secondary metabolites have often similar structure, the highest peak in two chromatograms were identified as acetate-1,2,3,4,4a,7,8,8a-octahydro-2,4a ,5,8a-tetramethyl-1-naphtha- lenyl ester. In addition, 6 main compounds in A and B were the same, which proved that they possessed the similar scent. However, their aroma quality was different because there were 5 unique decalin type compounds in A, which illustrated the top note of A was different from B. On the other hand, there were 14 distinctive compounds of longer retention time in B, which said that the oil B had longer holding time of fragrance than A. Furthermore, the SPME-GC-MS technique was an effective way to evaluate the quality of Agarwood essential oils according to the results comparing to the fragrance constituents in Agarwood essential oils extracted by 4 different extraction methods.