CAJ | 학술논문

本文建立了用反相液相色谱同时测定糖品中的苯甲酸、山梨酸、脱氢乙酸、安赛蜜、糖精钠等防腐剂和甜味剂的方法。方法采用VenusilXBP—C18（5gm，4．6x250mm）反相色谱柱，流动相为20mmol／L乙酸铵一甲醇，流速为1．0mL／min，柱温为30℃，进样量为10gL。该方法的测定低限为1．0mg／L，线性范围为10～100mg／L，加标回收率为89．85％～103．60％，相对标准偏差为1．50％～3．26％（n=4）。结果表明，该方法简便、可靠，适合对糖品中防腐剂和甜味剂的同时检测。
본문건립료용반상액상색보동시측정당품중적분갑산、산리산、탈경을산、안새밀、당정납등방부제화첨미제적방법。방법채용VenusilXBP—C18（5gm，4．6x250mm）반상색보주，류동상위20mmol／L을산안일갑순，류속위1．0mL／min，주온위30℃，진양량위10gL。해방법적측정저한위1．0mg／L，선성범위위10～100mg／L，가표회수솔위89．85％～103．60％，상대표준편차위1．50％～3．26％（n=4）。결과표명，해방법간편、가고，괄합대당품중방부제화첨미제적동시검측。
Reverse-phase HPLC was applied to the determination of benzoic acid, sorbic acid, dehydroacetic acid, acesulfame K, sodium saccharin in sugar. The analysis column was Venusil XBP - C18 (5 μm, 4.6×250 mm), the mobile phase was Methanol - 20 mmol/L NHgAc, the flow rate was 1.0 mL/min. The column temperature was 30 ℃, and the injection volume was 10 μL. The limit of quantitation was 1.0 mg/L. The linear plots were obtained between 10 and 100 mg/L. Overall recoveries were between 89.85% and 103.60%, and RSD (n = 4) value were 1.50% and 3.26%. Results showed this method was simple and accurate, it could be applied for determination of preservative and sweetener in sugar.