CAJ | 학술논문

建立同时检测菊花中7种化合物HPLC方法。采用乙腈-0.1%磷酸水溶液梯度洗脱,流速：0.8mL/min,进样量：10μL,检测波长：348nm,柱温：30℃,同时检测绿原酸、木犀草苷、3,5-0-二咖啡酰基奎宁酸、芦丁、黄芩苷、槲皮素和金合欢素等7种化合物。HPLC法图谱比较,发现6种不同菊花7种化合物成分峰面积比例具有很大差异。试验结果表明,该方法能够简便、灵敏、直观地揭示不同菊花的差异,为菊花的指纹图谱建立和菊花多成分的含量测定提供参考方法。
건립동시검측국화중7충화합물HPLC방법。채용을정-0.1%린산수용액제도세탈,류속：0.8mL/min,진양량：10μL,검측파장：348nm,주온：30℃,동시검측록원산、목서초감、3,5-0-이가배선기규저산、호정、황금감、곡피소화금합환소등7충화합물。HPLC법도보비교,발현6충불동국화7충화합물성분봉면적비례구유흔대차이。시험결과표명,해방법능구간편、령민、직관지게시불동국화적차이,위국화적지문도보건립화국화다성분적함량측정제공삼고방법。
To establish a method for determining 7 Kinds of Compounds in Flos Chrysanthemi,acetonitrile-0.1% phosphoric acid aqueous gradient elution,flow rate:0.8mL/min,sampling volume:10μL,detection wavelength:348nm,column temperature 30℃,simultaneous determination of caffeotannic acid,galuteolin,3,5-0-bis caffeoylquinic acid,rutin,baicalin,meletin and acacetin from seven Kinds of Compounds are used.Comparison of HPLC chromatograms has the founding that the peak area ratio of six different chrysanthemum from seven kinds of compound ingredients are very different.The results show that the method is simple,sensitive,direct-viewing,and reveal the differences of chrysanthemum.This provids a reference method for simultaneous determination of multicomponent in chrysanthemum flos and the establishment of fingerprint.