CAJ | 학술논문

以甜叶菊为试材,采用正交法对影响其相关序列扩增多态性（SRAP）反应体系的5个因子进行优化,进而对来自国内外的12个品种进行亲缘关系分析。结果表明,5个因素对SRAP反应体系影响大小顺序为：MgCl2,Primers,Taq,dNTPS和DNA。20μL的SRAP优化体系包括：双蒸水12.7μL,10×Buffer（含15mmol/L MgCl2）溶液3μL,2.5 mmol/L的dNTPS 0.8μL,10μmol/L的前后引物各0.75μL,20ng的模板DNA 1μL,5U Taq聚合酶;品种内遗传距离的变异范围为0.24～0.69,当遗传距离为0.48时,可将上述12个甜叶菊品种分为四类。
이첨협국위시재,채용정교법대영향기상관서렬확증다태성（SRAP）반응체계적5개인자진행우화,진이대래자국내외적12개품충진행친연관계분석。결과표명,5개인소대SRAP반응체계영향대소순서위：MgCl2,Primers,Taq,dNTPS화DNA。20μL적SRAP우화체계포괄：쌍증수12.7μL,10×Buffer（함15mmol/L MgCl2）용액3μL,2.5 mmol/L적dNTPS 0.8μL,10μmol/L적전후인물각0.75μL,20ng적모판DNA 1μL,5U Taq취합매;품충내유전거리적변이범위위0.24～0.69,당유전거리위0.48시,가장상술12개첨협국품충분위사류。
Optimization of 5 factors of sequence-related amplified polymorphism(SRAP) reaction system using the orthogonal design and relationship analysis of 12 kinds of cultivars in Stevia rebaudiana Bertoni were studied.The results indicated that the factor influencing SRAP system followed the order: MgCl2,Primers,Taq,dNTPS and DNA.The optimization system of 20μL included 12.7μL of ddH20,3μL of 10×Buffer with 15mmol/L MgCl2,0.8μL of 2.5 mmol/L dNTPS,0.75μL of 10μmol/L Primers,1μL of 20ng DNA and 5 unit of Taq polymerase.The result of clustering showed that the genetic distance among 12 cultivars ranged from 0.24 to 0.69,and could be classified into four groups when the genetic distance was 0.48.