CAJ | 학술논문

在510nm最大吸收光时检测正交提取蒲公英中的黄酮含量，建立正交提取工艺，对提取物进行HPLC检测，色谱柱为Eclipse×DB—C18柱（4．6mm×250mm，5μm），流动相为甲醇：1％冰醋酸：60：40（v／v），流速1．0mL／min，检测波长254nm，柱温30℃，进样量20μL，在此色谱条件下测定黄酮含量。结果表明，各因素影响力大小为：pH值〉浸泡时间〉料液比；用HPLC测定蒲公英中的黄酮含量为9．26％。
재510nm최대흡수광시검측정교제취포공영중적황동함량，건립정교제취공예，대제취물진행HPLC검측，색보주위Eclipse×DB—C18주（4．6mm×250mm，5μm），류동상위갑순：1％빙작산：60：40（v／v），류속1．0mL／min，검측파장254nm，주온30℃，진양량20μL，재차색보조건하측정황동함량。결과표명，각인소영향력대소위：pH치〉침포시간〉료액비；용HPLC측정포공영중적황동함량위9．26％。
Orthogonal extraction process for flavonoid from Herba Taraxaci was established by orthogonal test in 510 nm maximum optical absorption; Then the extract was detected by HPLC. The chromatographic condition was as follows: analytic column: Eclipse ×DB-C18 (4.6 mm × 250 mm, 5 μm), the mobile phase was methanol 1:1% glacial acetic acid = 60:40 (v/v), the flow rate was 1.0 mL/min, UV detection wavelength was 254 nm, column temperature was 30 ℃, aud sample size was 20 μL. The results showed that the factors influence was: pH value 〉 exlracting time 〉 solid to liquid ratio: the maximal flavonoid content in Herba Taraxaci was 9.26% detected by HPLC.