CAJ | 학술논문

研究胸腺五肽（TP5）溶液作为冻干疫苗稀释剂对疫苗免疫效果的影响。用不同浓度的TP5溶液稀释鸡新城疫Ⅳ系弱毒冻干苗，对ICR小鼠进行滴鼻免疫2次，间隔2周，并设立生理盐水对照组，分别于二免后1、2、3周眼眶采血并分离血清，采用间接ELISA方法测定总IgG水平、结果表明，与对照组相比，0．25—0．5mg／mL TP5溶液可显著提高二免后1周和2周小鼠血清中的抗体水平（P〈0．05）。提示TP5溶液可作为弱毒冻干疫苗的稀释液以增强疫苗的免疫效果。
연구흉선오태（TP5）용액작위동간역묘희석제대역묘면역효과적영향。용불동농도적TP5용액희석계신성역Ⅳ계약독동간묘，대ICR소서진행적비면역2차，간격2주，병설립생리염수대조조，분별우이면후1、2、3주안광채혈병분리혈청，채용간접ELISA방법측정총IgG수평、결과표명，여대조조상비，0．25—0．5mg／mL TP5용액가현저제고이면후1주화2주소서혈청중적항체수평（P〈0．05）。제시TP5용액가작위약독동간역묘적희석액이증강역묘적면역효과。
Effect of Thymopentin (TP5) solution as a diluent of freeze-dried attenuated vaccine on the immune response of animals was investigated. Different dosage of TP5 solution was used to dilute the Newcastle disease vaccine, and then inoculated intranasally to ICR mice twice at two weeks interval, a saline control was designed as well. Specific serum IgG antibody was detected by ELISA on 1, 2 and 3 weeks after the second immunization. The results showed that 0,25-0.5 mg/mL of TP5 could significantly increase the serum antibody level of mice on 1 and 2 weeks after the second immunization, which suggested that TP5 solution was a promising vaccine diluent for attenuated freeze-dried vaccine to enhance the immune response in animals.