CAJ | 학술논문

为研究ANXA2与结直肠癌发展的关系以及其在肿瘤发展过程中作用的构效关系，构建了ANXA2基因敲除质粒重组子，然后导入人结直肠癌细胞系Cac02，经过一系列筛选过程，最终建立了基因敲除ANXA2的Cac02细胞系（ANXA2-／-Cac02）．经过PCR和WesternBlotting检测，确认该细胞系无任何野生ANXA2表达．
위연구ANXA2여결직장암발전적관계이급기재종류발전과정중작용적구효관계，구건료ANXA2기인고제질립중조자，연후도입인결직장암세포계Cac02，경과일계렬사선과정，최종건립료기인고제ANXA2적Cac02세포계（ANXA2-／-Cac02）．경과PCR화WesternBlotting검측，학인해세포계무임하야생ANXA2표체．
To study the roles played by ANXA2 during the development of colorectal carcinoma, and the relationship between the structure and function of ANXA2, ANXA2 knockout recombinant was constructed and transfected into Caco2 cell line. After a series of selecting step, ANXA2 --/--Caco2 cell line was successfully generated. By the designated PCR and Western blot analysis, it was confirmed that the wild type ANXA2 expression is knockout completely in this cell line.