CAJ | 학술논문

以薯蓣粉为材料，设计4因素3水平的正交试验，酶法提取膳食纤维。结果表明：0．7％混合酶（晓一淀粉酶与糖化酶质量比为6：1），处理温度60℃，处理时间80rain，0．5％蛋白酶在60℃处理60min，对淀粉和蛋白质的脱除较彻底。以体外试验研究不同质量浓度（20-140mg／mL）的膳食纤维鲜样和可溶性淀粉对自由基的清除效果，设计3种不同的反应体系：1，1-二苯基-2-苦味肼基自由基（DPPH）、超氧阴离子自由基（O2＋）、羟自由基（·OH）。结果表明：薯蓣膳食纤维对3种自由基都有一定的清除能力，平均消除率分别为53．23％、31．67％、30．38％。对3种自由基清除能力大小顺序为：DPPH自由基〉超氧阴离子自由基〉羟自由基。另外，可溶性淀粉对3种自由基也有轻微的清除能力，其平均清除率分别为2．81％、6．98％、6．48％。
이서여분위재료，설계4인소3수평적정교시험，매법제취선식섬유。결과표명：0．7％혼합매（효일정분매여당화매질량비위6：1），처리온도60℃，처리시간80rain，0．5％단백매재60℃처리60min，대정분화단백질적탈제교철저。이체외시험연구불동질량농도（20-140mg／mL）적선식섬유선양화가용성정분대자유기적청제효과，설계3충불동적반응체계：1，1-이분기-2-고미정기자유기（DPPH）、초양음리자자유기（O2＋）、간자유기（·OH）。결과표명：서여선식섬유대3충자유기도유일정적청제능력，평균소제솔분별위53．23％、31．67％、30．38％。대3충자유기청제능력대소순서위：DPPH자유기〉초양음리자자유기〉간자유기。령외，가용성정분대3충자유기야유경미적청제능력，기평균청제솔분별위2．81％、6．98％、6．48％。
The dietary fiber was extracted from yam by enzymatic hydrolysis and tested by orthogonal ex- periment. The results indicated that the optimal extraction processing conditions were, 0.7% mixed en- zyme ( α - amylase and glucoamylase at the ratio of 6:1 ), hydrolysis temperature 60 ℃ and hydrolysis time 80 min, 0.5 % protease at 60 ℃ for 60 rain. The scavenging effects of fresh dietary fiber and solu- ble starch with various concentrations (20 -140 mg/mL) on DPPH, superoxide anion and hydroxyl free radicals were also investigated in vitro. Results exhibited that the dietary fiber had strong scavenging ca- pability on the 3 kinds of free radicals. The average scavenging rates of dietary fiber from yam on DPPH, superoxide anion and hydroxyl free radicals were 53.23% ,31.67% and 30.38%, respectively. In addi- tion, soluble starch had mild scavenging faction on the 3 kinds of free radicals. The average scavenging rates were 2.81% ,6.98% and 6.48%, respectively.