农业研究与应用
農業研究與應用
농업연구여응용
GUANGXI TROPICAL AGRICULTURE
2012年
6期
29-32
,共4页
液相色谱质谱法%植物性蛋白%三聚氰胺
液相色譜質譜法%植物性蛋白%三聚氰胺
액상색보질보법%식물성단백%삼취청알
Liquid chromatography-mass spectrometry%plant protein%melamine
建立三重四极杆液相色谱质谱联用分析植物性蛋白中三聚氰胺的检测方法。方法：样品用1%乙酸＋乙腈（4∶1,V/V）提取,固相萃取柱净化,利用液质联用的多反应监测（MRM）技术,对三聚氰胺进行定性定量分析,以三聚氰胺的离子对m/z127/65进行定性,以离子对m/z127/85进行定量。结果：方法在0.025μg/mL～0.400μg/mL的范围内呈良好的线性关系,加标平均回收率为87.2%～99.9%,试验的RSD为0.51%～1.23%。结论：该方法通过优化仪器分析条件,操作方便,重现性好,回收率高,结果准确,为三聚氰胺的检测提供了准确可靠的方法。
建立三重四極桿液相色譜質譜聯用分析植物性蛋白中三聚氰胺的檢測方法。方法：樣品用1%乙痠＋乙腈（4∶1,V/V）提取,固相萃取柱淨化,利用液質聯用的多反應鑑測（MRM）技術,對三聚氰胺進行定性定量分析,以三聚氰胺的離子對m/z127/65進行定性,以離子對m/z127/85進行定量。結果：方法在0.025μg/mL～0.400μg/mL的範圍內呈良好的線性關繫,加標平均迴收率為87.2%～99.9%,試驗的RSD為0.51%～1.23%。結論：該方法通過優化儀器分析條件,操作方便,重現性好,迴收率高,結果準確,為三聚氰胺的檢測提供瞭準確可靠的方法。
건립삼중사겁간액상색보질보련용분석식물성단백중삼취청알적검측방법。방법：양품용1%을산＋을정（4∶1,V/V）제취,고상췌취주정화,이용액질련용적다반응감측（MRM）기술,대삼취청알진행정성정량분석,이삼취청알적리자대m/z127/65진행정성,이리자대m/z127/85진행정량。결과：방법재0.025μg/mL～0.400μg/mL적범위내정량호적선성관계,가표평균회수솔위87.2%～99.9%,시험적RSD위0.51%～1.23%。결론：해방법통과우화의기분석조건,조작방편,중현성호,회수솔고,결과준학,위삼취청알적검측제공료준학가고적방법。
An inspection method of triple quadruple liquid chromatography-mass spectrometry was developed for the determination ofmela~nine in plant protein. Methods: samples were extracted with 1% acetic acid + acetonitrile (4:1, V / V), and purified through a solid phase extraction col- umn. Qualitative and quantitative analysis of melamine was performed by using the multiple reaction monitoring (MRM)technology of liquid chromatography-mass spectrometry, and the ion pairs m/z127〉 65 and m/z127〉 85 of melamine were used for qualification and quantification respectively. Results: the developed method presented gooO linearity in the range of 0.025 μg/mL to 0.4001μg/mL. The average recoveries were 87.2 % to 99.9 %, and the RSD was 0.51% to 1.23 %. Conclusion: by using the optimal instruments, the method has the advantages of easy operation, good reproducibility, high recovery rate and accurate results for the detection of melamine.
