CAJ | 학술논문

为了更好地指导养殖户应用高致病性蓝耳病活疫苗（JXA1-R株），本中心从淋巴细胞计数、病毒核酸检测、免疫抗体检测等方面，分析了高致病性蓝耳病弱毒活疫苗免疫应答情况。本次试验选取30天龄的杜长大三元断奶仔猪20头，随机分成A、B两组，每组10头。A组注射JXA1-R株活疫苗，B组头注射生理盐水作对照。结果显示该疫苗免疫仔猪28天后，淋巴细胞由7．585×10^9／L上升到15．28×10^9／L，免疫抗体阳性率由0％上升到100％，免疫猪病毒核酸检出率由0％上升到90％。揭示了PRRS活疫苗（JXA1-R株）能使仔猪产生较好的免疫应答，疫苗毒感染持续时间多数超过28天。
위료경호지지도양식호응용고치병성람이병활역묘（JXA1-R주），본중심종림파세포계수、병독핵산검측、면역항체검측등방면，분석료고치병성람이병약독활역묘면역응답정황。본차시험선취30천령적두장대삼원단내자저20두，수궤분성A、B량조，매조10두。A조주사JXA1-R주활역묘，B조두주사생리염수작대조。결과현시해역묘면역자저28천후，림파세포유7．585×10^9／L상승도15．28×10^9／L，면역항체양성솔유0％상승도100％，면역저병독핵산검출솔유0％상승도90％。게시료PRRS활역묘（JXA1-R주）능사자저산생교호적면역응답，역묘독감염지속시간다수초과28천。
Lymphocytes, virus nucleic acid and immune antibody were detected in our lab on analyzing immune response against attenuated live vaccine on highly pathogenic Porcine Reproductive and Respiratory Syndrome (PRRS) for guiding applications of this vaccine by farmers betterly. Twenty 30-day-old ternary weaned piglets were randomly divided into two groups A and B. The pigs in Group A were injected with live vaccine and the pigs in group B were injected with saline solution as control. The results showed that the Lymphocytes increased from 7.585 × 10^9/L to 15.28 × 10^9/L, the antibody-positive rate increased from 0% to 100%, virus nucleic acid positive rate increased from 0% to 90% in vaccined piglets after 28 days. The results showed that the live vaccine(JXA1-R strain) of PRRS could induce better immune response in pigs and the duration time of vaccine virus infection were more than 28 days.