农学学报
農學學報
농학학보
Chinese Countryside Well-off Technology
2012年
10期
55-57,67
,共4页
葡萄脱毒苗木%离体快繁%有效苗%继代增殖培养基%生根培养基
葡萄脫毒苗木%離體快繁%有效苗%繼代增殖培養基%生根培養基
포도탈독묘목%리체쾌번%유효묘%계대증식배양기%생근배양기
Grapes Detoxification Seedlings%Vitro Rapid Propagation%Effective Seedlings%SubcultureProliferation Medium%Rooting Culture Medium
为了培育葡萄脱毒苗木,更进一步促进葡萄组织培养技术的研究,对影响葡萄离体快繁的若干因素进行试验,选出最适葡萄离体快繁的培养基。取不同状态（休眠期、生长期）的2年生枝条作为材料,消毒后在超净工作台上切成1.5cm带有腋芽的茎段进行培养对比,发现春季即将解除休眠的枝条上的冬芽发芽率和成活率最高、生长势最好。继代增殖培养基MS＋6-BA 1.0mg/L＋IBA 0.1mg/L的组合生长的有效苗最多,生根培养基为1/2MS＋IBA 0.4mg/L,IBA最有利于葡萄的生根,这个浓度中的苗炼苗的成活率最高。在试验过程中最佳增殖组合中偶尔也会有玻璃苗出现,所以此次试验得到的最佳继代培养基只是在这几种浓度中的最好配比组合,应将浓度在现有的最佳配比范围上下浮动进行更进一步的研究,而对于生根培养也可尝试用1/4MS、1/6MS、1/8MS进行进一步的试验研究。
為瞭培育葡萄脫毒苗木,更進一步促進葡萄組織培養技術的研究,對影響葡萄離體快繁的若榦因素進行試驗,選齣最適葡萄離體快繁的培養基。取不同狀態（休眠期、生長期）的2年生枝條作為材料,消毒後在超淨工作檯上切成1.5cm帶有腋芽的莖段進行培養對比,髮現春季即將解除休眠的枝條上的鼕芽髮芽率和成活率最高、生長勢最好。繼代增殖培養基MS＋6-BA 1.0mg/L＋IBA 0.1mg/L的組閤生長的有效苗最多,生根培養基為1/2MS＋IBA 0.4mg/L,IBA最有利于葡萄的生根,這箇濃度中的苗煉苗的成活率最高。在試驗過程中最佳增殖組閤中偶爾也會有玻璃苗齣現,所以此次試驗得到的最佳繼代培養基隻是在這幾種濃度中的最好配比組閤,應將濃度在現有的最佳配比範圍上下浮動進行更進一步的研究,而對于生根培養也可嘗試用1/4MS、1/6MS、1/8MS進行進一步的試驗研究。
위료배육포도탈독묘목,경진일보촉진포도조직배양기술적연구,대영향포도리체쾌번적약간인소진행시험,선출최괄포도리체쾌번적배양기。취불동상태（휴면기、생장기）적2년생지조작위재료,소독후재초정공작태상절성1.5cm대유액아적경단진행배양대비,발현춘계즉장해제휴면적지조상적동아발아솔화성활솔최고、생장세최호。계대증식배양기MS＋6-BA 1.0mg/L＋IBA 0.1mg/L적조합생장적유효묘최다,생근배양기위1/2MS＋IBA 0.4mg/L,IBA최유리우포도적생근,저개농도중적묘련묘적성활솔최고。재시험과정중최가증식조합중우이야회유파리묘출현,소이차차시험득도적최가계대배양기지시재저궤충농도중적최호배비조합,응장농도재현유적최가배비범위상하부동진행경진일보적연구,이대우생근배양야가상시용1/4MS、1/6MS、1/8MS진행진일보적시험연구。
In order to cultivate grapes detoxification seedlings and further promote the study of grape tissue culture techniques, a number of factors affecting the vitro rapid propagation of grapes were tested and the culture medium most suitable for the grape in vitro rapid propagation was elected. 2-year-old branches at different states (dormant period, growing season) were taken as the material which was cut into 1.5 cm stem segments after disinfection in the ultra-clean work bench to be cultured to contrast. It was found that in spring, the winter buds of the branches after which were relieved dormancy had the highest sprout rate and survival rate and the best growth potential. The combination of growth of the culture medium of subculture multiplication MS+6-BA 1.0 mg/L+ IBA 0.1 mg/L had the maximum effective seedlings. The rooting culture medium, 1/2MS + IBA 0.4 mg/L, IBA was the most conducive to the rooting of grape, the seeding in this concentration had the highest survival rate. In the process of the experiment, the vitrification seeds occasionally appeared in the optimum proliferation combination, therefore, the best subculture medium obtained in the experiment was just the best proportioning combination. A further study on that the concentration is adjusted plus or minus within the range of the best proportioning should be done. For rooting culture, 1/4MS, 1/6MS, 1/8MS try to be used for further nilot studv_
