CAJ | 학술논문

试验比较了MA-104、Marc-145、Vero3种猴肾细胞增殖犬瘟热病毒的敏感性，按常规方法将犬瘟热病毒（CDV—XN112株）分别接种于MA-104、Marc-145、Vero3种细胞，通过观察细胞病变，确定收毒时间，比较病毒滴度。结果表明，犬瘟热病毒可在MA-104、Marc-145、Vero3种细胞内增殖，并出现典型细胞病变，效价均达到10^4．5TCID50／0．1mL以上．而Vero细胞增殖犬瘟热病毒更为经济高效，是增殖该病毒的理想细胞。
시험비교료MA-104、Marc-145、Vero3충후신세포증식견온열병독적민감성，안상규방법장견온열병독（CDV—XN112주）분별접충우MA-104、Marc-145、Vero3충세포，통과관찰세포병변，학정수독시간，비교병독적도。결과표명，견온열병독가재MA-104、Marc-145、Vero3충세포내증식，병출현전형세포병변，효개균체도10^4．5TCID50／0．1mL이상．이Vero세포증식견온열병독경위경제고효，시증식해병독적이상세포。
After canine distemper virus (CDV-XN 112 strain) was inoculated into MA- 104, Marc- 145 and Vero cells, the sensitivity of three types of monkey kidney to CDV was compared by observing cell pathological effect, determining the time for harvesting virus and comparing virus titers. The results showed that three cells were suitable for CDV, with typical cell pathological effect, and the titers of virus harvest fluids were more than 104.5 TCID50/0. lmL, among them Vero cell was more cost-effective and ideal for proliferation CDV.