CAJ | 학술논문

为研究牦牛肌肉中脂肪酸组成特征及其形成机制，以牦牛和黄牛背最长肌为材料，利用气象色谱-质谱联用仪( GC-MS)进行脂肪酸的定性定量分析，并通过Real-time PCR技术检测FASN基因在牦牛和黄牛背最长肌中mRNA的表达水平，同时分析了其与脂肪酸组成的相关性。结果发现，牦牛背最长肌中单不饱和脂肪酸( MUFA)和多不饱和脂肪酸(PUFA)含量均极显著高于黄牛(P<0.01)，而饱和脂肪酸(SFA)显著低于黄牛(P<0.05)。牦牛背最长肌中FASN基因的mRNA表达水平极显著高于黄牛(P<0.01)。牦牛FASN基因mRNA表达量只与牦牛C10：0呈极显著正相关(P<0.01)，与黄牛脂肪酸组成无显著的相关性(P>0.05)。
위연구모우기육중지방산조성특정급기형성궤제，이모우화황우배최장기위재료，이용기상색보-질보련용의( GC-MS)진행지방산적정성정량분석，병통과Real-time PCR기술검측FASN기인재모우화황우배최장기중mRNA적표체수평，동시분석료기여지방산조성적상관성。결과발현，모우배최장기중단불포화지방산( MUFA)화다불포화지방산(PUFA)함량균겁현저고우황우(P<0.01)，이포화지방산(SFA)현저저우황우(P<0.05)。모우배최장기중FASN기인적mRNA표체수평겁현저고우황우(P<0.01)。모우FASN기인mRNA표체량지여모우C10：0정겁현저정상관(P<0.01)，여황우지방산조성무현저적상관성(P>0.05)。
In order to study the characteristics and mechanism of fatty acid composition in yak,the fatty acids composition and content of Longissimus dorsi muscle in yak and cattle were determined by using gas chromatograph-mass spectrometer-computer( GC-MS) ,while the FASN gene mRNA expression level was investigated by Real-time PCR,and the correlation analysis was carried out. The results showed that the monounsaturated fatty acid( MUFA) and polyunsaturated fatty acids(PUFA) in yak was extremely significantly higher than in cattle(P< 0. 01),while the saturated fatty acid(SFA) was significantly lower than in cattle(P< 0. 05) . The FASN mRNA expression in yak was extremely significantly higher than in cattle(P< 0. 01) . There is an extremely significant positive correla-tion(P< 0. 01) between the FASN mRNA expression level and content of C10:0 in yak,no significant correlation (P>0. 05) was found in cattle.