CAJ | 학술논문

建立了一种用高效液相色谱仪分析辣椒和土壤中的申嗪霉素残留量方法。样品以丙酮和冰醋酸混合溶液提取,辣椒样品过SPE小柱净化,土壤直接浓缩、定容,最后用液相色谱DAD检测器测定。该方法条件下,申嗪霉素的最小检出量为：0.2ng,在辣椒和土壤中最低检测浓度分别为0.001mg/kg,申嗪霉素在样品中的回收率为78.9～101.8%,变异系数为0.99～8.29%,满足农药残留分析要求。
건립료일충용고효액상색보의분석랄초화토양중적신진매소잔류량방법。양품이병동화빙작산혼합용액제취,랄초양품과SPE소주정화,토양직접농축、정용,최후용액상색보DAD검측기측정。해방법조건하,신진매소적최소검출량위：0.2ng,재랄초화토양중최저검측농도분별위0.001mg/kg,신진매소재양품중적회수솔위78.9～101.8%,변이계수위0.99～8.29%,만족농약잔류분석요구。
A high-performance liquid chromatography(HPLC)method was developed for the determination of phenazine-1-carboxylic acid in capsicum and soil.The residues of sample were extracted with acetone(or acetonitrile)and acetic acid mixture,and capsicum sample were cleaned up with Solid-phase extraction,and condensed to dry.Determinations of analytes were completed using HPLC with DAD detection at 250nm.Under HPLC conditions,the detectable limit of phenazine-1-carboxylic acid was estimated to be 0.2ng,and the minimum determination concentration was 0.001mg/kg in capsicum and soil respectively,The fortified recoveries of phenazine-1-carboxylic acid in straw,chaff and unpolished grain were in range of 78.9~101.8%,with relative standard deviations of 0.99~8.29%.