中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2014年
3期
219-224
,共6页
卵巢肿瘤%Wnt2B%PAI-1%免疫组织化学
卵巢腫瘤%Wnt2B%PAI-1%免疫組織化學
란소종류%Wnt2B%PAI-1%면역조직화학
Ovarian neoplasms%Wnt2B%PAI-1%Immunohistochemistry
目的 通过检测Wnt2B与PAI-1在上皮性卵巢癌中的表达,分析两者与上皮性卵巢癌侵袭转移及临床病理之间的关系,以探讨两种基因在上皮性卵巢癌的表达及临床意义.方法 应用免疫组化(SP法)检测2008年1月到2013年6月在本院行手术切除的上皮性卵巢癌82例和良性卵巢上皮瘤10例患者组织中Wnt2B和PAI-1蛋白的表达情况,分析上皮性卵巢癌的年龄、组织学类型、细胞学分级、手术病理分期、肿瘤病灶大小、盆腔转移灶大小、大网膜转移、淋巴结转移、远处转移、残留病灶大小等临床病理因素与两个基因的表达关系;分析上皮性卵巢癌患者癌组织中Wnt2B与PAI-1的表达关系.结果 上皮性卵巢癌患者癌组织中Wnt2B的阳性表达率为12.2％(10/82),低于良性卵巢上皮瘤患者瘤组织的70％ (7/10)(P＜0.05);上皮性卵巢癌患者癌组织中PAI-1的阳性表达率为76.8％(63/82),高于良性卵巢上皮瘤患者瘤组织的20％ (2/10) (P＜0.05).PAI-1的表达与手术病理分期、细胞学分级、大网膜转移、盆腔转移灶大小有关(均P＜0.05),而与患者年龄、组织学类型、肿瘤病灶大小、淋巴结转移、远处转移、残留病灶大小无关(均P＞0.05);Wnt2B表达与手术病理分期、大网膜转移有关(均P＜0.05),与患者年龄、组织学类型、细胞学分级、肿瘤病灶大小、盆腔转移灶大小、淋巴结转移、远处转移、残留病灶大小无关(均P＞0.05).上皮性卵巢癌患者癌组织中Wnt2B与PAI-1的表达呈负相关(r=-0.847,P＜0.05).结论 Wnt2B在上皮性卵巢癌的发病机制中可能发挥一定的作用.PAI-1的表达与上皮性卵巢癌的发生、发展、浸润、转移有关.
目的 通過檢測Wnt2B與PAI-1在上皮性卵巢癌中的錶達,分析兩者與上皮性卵巢癌侵襲轉移及臨床病理之間的關繫,以探討兩種基因在上皮性卵巢癌的錶達及臨床意義.方法 應用免疫組化(SP法)檢測2008年1月到2013年6月在本院行手術切除的上皮性卵巢癌82例和良性卵巢上皮瘤10例患者組織中Wnt2B和PAI-1蛋白的錶達情況,分析上皮性卵巢癌的年齡、組織學類型、細胞學分級、手術病理分期、腫瘤病竈大小、盆腔轉移竈大小、大網膜轉移、淋巴結轉移、遠處轉移、殘留病竈大小等臨床病理因素與兩箇基因的錶達關繫;分析上皮性卵巢癌患者癌組織中Wnt2B與PAI-1的錶達關繫.結果 上皮性卵巢癌患者癌組織中Wnt2B的暘性錶達率為12.2％(10/82),低于良性卵巢上皮瘤患者瘤組織的70％ (7/10)(P＜0.05);上皮性卵巢癌患者癌組織中PAI-1的暘性錶達率為76.8％(63/82),高于良性卵巢上皮瘤患者瘤組織的20％ (2/10) (P＜0.05).PAI-1的錶達與手術病理分期、細胞學分級、大網膜轉移、盆腔轉移竈大小有關(均P＜0.05),而與患者年齡、組織學類型、腫瘤病竈大小、淋巴結轉移、遠處轉移、殘留病竈大小無關(均P＞0.05);Wnt2B錶達與手術病理分期、大網膜轉移有關(均P＜0.05),與患者年齡、組織學類型、細胞學分級、腫瘤病竈大小、盆腔轉移竈大小、淋巴結轉移、遠處轉移、殘留病竈大小無關(均P＞0.05).上皮性卵巢癌患者癌組織中Wnt2B與PAI-1的錶達呈負相關(r=-0.847,P＜0.05).結論 Wnt2B在上皮性卵巢癌的髮病機製中可能髮揮一定的作用.PAI-1的錶達與上皮性卵巢癌的髮生、髮展、浸潤、轉移有關.
목적 통과검측Wnt2B여PAI-1재상피성란소암중적표체,분석량자여상피성란소암침습전이급림상병리지간적관계,이탐토량충기인재상피성란소암적표체급림상의의.방법 응용면역조화(SP법)검측2008년1월도2013년6월재본원행수술절제적상피성란소암82례화량성란소상피류10례환자조직중Wnt2B화PAI-1단백적표체정황,분석상피성란소암적년령、조직학류형、세포학분급、수술병리분기、종류병조대소、분강전이조대소、대망막전이、림파결전이、원처전이、잔류병조대소등림상병리인소여량개기인적표체관계;분석상피성란소암환자암조직중Wnt2B여PAI-1적표체관계.결과 상피성란소암환자암조직중Wnt2B적양성표체솔위12.2％(10/82),저우량성란소상피류환자류조직적70％ (7/10)(P＜0.05);상피성란소암환자암조직중PAI-1적양성표체솔위76.8％(63/82),고우량성란소상피류환자류조직적20％ (2/10) (P＜0.05).PAI-1적표체여수술병리분기、세포학분급、대망막전이、분강전이조대소유관(균P＜0.05),이여환자년령、조직학류형、종류병조대소、림파결전이、원처전이、잔류병조대소무관(균P＞0.05);Wnt2B표체여수술병리분기、대망막전이유관(균P＜0.05),여환자년령、조직학류형、세포학분급、종류병조대소、분강전이조대소、림파결전이、원처전이、잔류병조대소무관(균P＞0.05).상피성란소암환자암조직중Wnt2B여PAI-1적표체정부상관(r=-0.847,P＜0.05).결론 Wnt2B재상피성란소암적발병궤제중가능발휘일정적작용.PAI-1적표체여상피성란소암적발생、발전、침윤、전이유관.
Objective To determine the expression of Wnt2B and plasminogen activator inhibitor-1 (PAI-1) in epithelial ovarian carcinoma (EOC) and analyze their relationships with the clinicopathology and metastasis of EOC for investigating the expression and clinical significance of the two genes in EOC.Methods Expressions of Wnt2B and PAI-1 were detected by immunhistochemical staining (SP) in 82 cases of EOC and 10 cases of benign ovarian tumor undergoing operation in our hospital from Jauary 2008 to June 2013.Association of these expressions with clinicopathologic factors such as age,histological type,cytological grading,surgical pathological stage,tumor size,size of pelvic metastasis locus,greater omentum metastasis,lymph node metastasis,distant metastasis,residual locus size of EOC was examined.And the relationship between Wnt2B expression and PAI-1 expression in cancer tissues was analyzed as well.Results The positive rate of Wnt2B expression in EOC was 12.2％ (10/82),which was lower than 70％ in benign ovarian tumor (7/10) (P＜0.05).The positive rate of PAI-1 in EOC was 76.8％ (63/82),which was higher than 20％ in benign ovarian tumor (2/10) (P＜0.05).The expression of PAI-1 in EOC was related to surgical pathological stages,cytological grading,greater omentum metastasis and the size of pelvic metastasis locus (all P＜0.05),but was not related to the age,pathological type,tumor size,lymph node metastasis,distant metastasis,or residual locus size (allP＞0.05).The expression of Wnt2B was related to surgical pathological stages and greater omentum metastasis (all P＜0.05),but was not related to the age,pathological type,cytological grading,tumor size,size of pelvic metastasis locus,lymph node metastasis,distant metastasis or residual locus size (all P＞0.05).Wnt2B expression was negatively correlated to PAI-1 expression in EOC (r=-0.847,P＜0.05).Conclusions Wnt2B may play a certain role in the pathogenesis of EOC.The expression of PAI-1 is related to the occurrence,development,invasion,metastasis in EOC.
