CAJ | 학술논문

测定细基丸叶和茎中总三萜的含量。以乌苏酸为对照品,以质量分数5%香草醛-冰醋酸和高氯酸系统为显色剂,采用紫外分光光度法在546 nm波长处测定样品的吸光度。乌苏酸对照品在20～100μg/mL范围内呈良好线性关系,回归方程为Abs=0.1548C＋0.0286,r=0.9993。细基丸叶和茎中总三萜的含量分别为1.684%和0.5439%.本法操作简便,结果稳定,重复性好,易于推广。
측정세기환협화경중총삼첩적함량。이오소산위대조품,이질량분수5%향초철-빙작산화고록산계통위현색제,채용자외분광광도법재546 nm파장처측정양품적흡광도。오소산대조품재20～100μg/mL범위내정량호선성관계,회귀방정위Abs=0.1548C＋0.0286,r=0.9993。세기환협화경중총삼첩적함량분별위1.684%화0.5439%.본법조작간편,결과은정,중복성호,역우추엄。
To determine the content of the tritepenoidic acid in the leaves and the stems of Polyalthia plagioneura Diels,the experimental conditions including ursolic acid as the reference,5% vanillin-acetic acid and perchloric acid as color system,the content of triterpenoidic acid in the leaves and stems of Polyalthia plagioneura was determined by ultraviolet spectrophotometric method at 546 nm.The calibration curve was linear(r=0.9993) in the range of 0.2~1.6 μg/mL,and the curve was Abs=0.1548C+0.0286,the content was 1.684% and 0.5439%.The result was stable,and had good repeatability.This method operated conveniently and spread easily.