广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2012年
22期
84-85,100
,共3页
胰酶%胰激肽原酶%胰蛋白酶%分离纯化
胰酶%胰激肽原酶%胰蛋白酶%分離純化
이매%이격태원매%이단백매%분리순화
trypsogen power%kallikein%trypsin%purification
研究了利用胰酶粉提取胰激肽释放酶的动态提取工艺。分别考察了醋酸钠添加与否、洗脱的速率、不同高径比的层析柱,梯度洗脱与直接洗脱,一次梯度洗脱与二次梯度洗脱等因素对利用胰酶粉提取胰激肽释放酶的影响。利用胰酶粉提取胰激肽释放酶的动态洗脱胰激肽释放酶的最佳条件为：控制流速为2 mL/min,添加醋酸钠,梯度洗脱及一次洗脱。本实验考察的动态条件为进一步胰激肽释放酶的分离纯化提供了理论依据。
研究瞭利用胰酶粉提取胰激肽釋放酶的動態提取工藝。分彆攷察瞭醋痠鈉添加與否、洗脫的速率、不同高徑比的層析柱,梯度洗脫與直接洗脫,一次梯度洗脫與二次梯度洗脫等因素對利用胰酶粉提取胰激肽釋放酶的影響。利用胰酶粉提取胰激肽釋放酶的動態洗脫胰激肽釋放酶的最佳條件為：控製流速為2 mL/min,添加醋痠鈉,梯度洗脫及一次洗脫。本實驗攷察的動態條件為進一步胰激肽釋放酶的分離純化提供瞭理論依據。
연구료이용이매분제취이격태석방매적동태제취공예。분별고찰료작산납첨가여부、세탈적속솔、불동고경비적층석주,제도세탈여직접세탈,일차제도세탈여이차제도세탈등인소대이용이매분제취이격태석방매적영향。이용이매분제취이격태석방매적동태세탈이격태석방매적최가조건위：공제류속위2 mL/min,첨가작산납,제도세탈급일차세탈。본실험고찰적동태조건위진일보이격태석방매적분리순화제공료이론의거。
The dynamic extraction experiment of kallikein from trypsogen power was studied.The addition of CH3COONa,the rate of elution,different height to diameter ratio of column chromatography,gradient elution or straight elution,the time of gradient elution on the use of trypsogen power orthogonal static extraction of pancreatic kallikein were investigated.The optimal dynamic kallikein extracted conditions were the flow rate controlled at 2 mL/min,added CH3COONa,and one time of gradient elution.The effect of dynamic conditions was to provide a theoretical basis for separation and purification of kallikein.
