金陵科技学院学报
金陵科技學院學報
금릉과기학원학보
JOURNAL OF JINLING INSTITUTE OF TECHNOLOGY
2012年
2期
86-89
,共4页
核苷酸%抗氧化%免疫
覈苷痠%抗氧化%免疫
핵감산%항양화%면역
nucleotides%antioxidant%immune
旨在研究外源性核苷酸对产蛋后期蛋鸡抗氧化作用及免疫机能的影响。选取体重均一的450日龄产蛋后期蛋鸡80只,随机分为对照组（C）和实验组（E）。两组在前期饲喂正常日粮的基础上添加0.25%核苷酸,450日龄时C组继续前期的核苷酸添加,E组停喂核苷酸。停喂15、45d后两组分别随机选取10只,处死并收集血液及器官组织样品进行相关指标的测定。结果显示：停喂15d时,实验组肝脏、肾脏中MDA含量,肝脏、肾脏、心脏、输卵管中IL-1β含量,肝脏、肾脏中TNF-α含量都显著高于对照组（P〈0.05）;停喂45d时,实验组肝脏、肾脏、十二指肠中MDA含量,肝脏、肾脏、心脏中IL-1β含量,肝脏、心脏中TNF-α含量都显著高于对照组（P〈0.05）。组织中IL-2含量以及15d输卵管中T-AOC变化刚好相反。说明外源性核苷酸对产蛋后期蛋鸡组织器官具有抗氧化以及调节免疫作用。
旨在研究外源性覈苷痠對產蛋後期蛋鷄抗氧化作用及免疫機能的影響。選取體重均一的450日齡產蛋後期蛋鷄80隻,隨機分為對照組（C）和實驗組（E）。兩組在前期飼餵正常日糧的基礎上添加0.25%覈苷痠,450日齡時C組繼續前期的覈苷痠添加,E組停餵覈苷痠。停餵15、45d後兩組分彆隨機選取10隻,處死併收集血液及器官組織樣品進行相關指標的測定。結果顯示：停餵15d時,實驗組肝髒、腎髒中MDA含量,肝髒、腎髒、心髒、輸卵管中IL-1β含量,肝髒、腎髒中TNF-α含量都顯著高于對照組（P〈0.05）;停餵45d時,實驗組肝髒、腎髒、十二指腸中MDA含量,肝髒、腎髒、心髒中IL-1β含量,肝髒、心髒中TNF-α含量都顯著高于對照組（P〈0.05）。組織中IL-2含量以及15d輸卵管中T-AOC變化剛好相反。說明外源性覈苷痠對產蛋後期蛋鷄組織器官具有抗氧化以及調節免疫作用。
지재연구외원성핵감산대산단후기단계항양화작용급면역궤능적영향。선취체중균일적450일령산단후기단계80지,수궤분위대조조（C）화실험조（E）。량조재전기사위정상일량적기출상첨가0.25%핵감산,450일령시C조계속전기적핵감산첨가,E조정위핵감산。정위15、45d후량조분별수궤선취10지,처사병수집혈액급기관조직양품진행상관지표적측정。결과현시：정위15d시,실험조간장、신장중MDA함량,간장、신장、심장、수란관중IL-1β함량,간장、신장중TNF-α함량도현저고우대조조（P〈0.05）;정위45d시,실험조간장、신장、십이지장중MDA함량,간장、신장、심장중IL-1β함량,간장、심장중TNF-α함량도현저고우대조조（P〈0.05）。조직중IL-2함량이급15d수란관중T-AOC변화강호상반。설명외원성핵감산대산단후기단계조직기관구유항양화이급조절면역작용。
The present study investigates the effect of feeding exogenous nucleotides on antioxi- dant and immune activity of old laying hens. 80 old laying hens of 450 days are randomly divid- ed into two groups ： the control group and the experimental group. They are fed with basal diet supplemented with 0. 25M nucleotides before the experiment. When they are 450 days, the control group are fed as before, while the experimental group are stopped feeding nucleotides. 10 old laying hens from each group are randomly slaughtered to collect blood and tissue sam- ples after 15 d and 45 d respectively. The samples are determined by kit. The results show that on the 15th day, the contents of MDA in liver and kidney, IL-1β in liver, kidney, heart and oviduct, TNF-α in liver and kidney of the experimental group are higher compared with that of the control group （P〈0.05）. On the 45th day, the contents of MDA in liver, kidney and duo- denum, IL-1β in liver, kidney and heart, TNF-α in liver and heart of the experimental group are higher compared with that of the control group （P〈0. 05）. The contents of IL-2 in tissues and T-AOC in oviduct on the 15th day of the experimental group are reversed. Therefore, ex- ogenous nucleotides can develop honantioxidant and immune activity of old laying hens.
