重庆理工大学学报:自然科学
重慶理工大學學報:自然科學
중경리공대학학보:자연과학
Journal of Chongqing Institute of Technology
2012年
5期
24-27
,共4页
菌种鉴定%细菌%常规鉴定
菌種鑒定%細菌%常規鑒定
균충감정%세균%상규감정
species identification%bacteria%conventional identification
用常规鉴定和Biolog快速鉴定2种方法对分离得到的3株产脂肪酶菌株进行了分类鉴定。根据其菌落、菌体及生理生化特征,3株产脂肪酶菌株中CDCH110、CDCH111属于不动杆菌属,CDCH112属于假单胞菌属。Biolog快速鉴定结果显示:CDCH110为溶血不动杆菌;CDCH111为醋酸不动杆菌;CDCH112为产碱假单胞菌。通过对比分析,结果表明:2种方法得到的结果一致,但常规方法只能鉴定到属,Biolog快速鉴定可以鉴定到种;Biolog快速鉴定法具有快速、准确等优点。
用常規鑒定和Biolog快速鑒定2種方法對分離得到的3株產脂肪酶菌株進行瞭分類鑒定。根據其菌落、菌體及生理生化特徵,3株產脂肪酶菌株中CDCH110、CDCH111屬于不動桿菌屬,CDCH112屬于假單胞菌屬。Biolog快速鑒定結果顯示:CDCH110為溶血不動桿菌;CDCH111為醋痠不動桿菌;CDCH112為產堿假單胞菌。通過對比分析,結果錶明:2種方法得到的結果一緻,但常規方法隻能鑒定到屬,Biolog快速鑒定可以鑒定到種;Biolog快速鑒定法具有快速、準確等優點。
용상규감정화Biolog쾌속감정2충방법대분리득도적3주산지방매균주진행료분류감정。근거기균락、균체급생리생화특정,3주산지방매균주중CDCH110、CDCH111속우불동간균속,CDCH112속우가단포균속。Biolog쾌속감정결과현시:CDCH110위용혈불동간균;CDCH111위작산불동간균;CDCH112위산감가단포균。통과대비분석,결과표명:2충방법득도적결과일치,단상규방법지능감정도속,Biolog쾌속감정가이감정도충;Biolog쾌속감정법구유쾌속、준학등우점。
Three microbe with lipase activity, isolated tional identification methods and Biolog Microstation Sy by ste our laboratory, were identified by conven- m. The results from conventional identifica- tion showed that microbe CDCH110, CDCH111 all belonged to Acinetobacter sp. , and CDCH112 be- longed to Pseudomonas sp.. It indicated that CDCHll0 was Acinetobacter calcoaceticus, CDCHll 1 was Acinetobacter haemolyticus, and CDCH111 was Pseudomonas alcaligenes with Biolog Microstation Sy of stem. The results from two methods showed a well consistency. However, it could only get the genus microbe by the way of conventional identification methods, and the Biolog system could get the spe- cies. In addition, Biolog Microstation System has high accuracy