CAJ | 학술논문

本实验利用ISSR分子标记技术研究5个中国水仙（Narcissus tazetta var．chinensis Boem＆Chen）居群的遗传多样性。从40个随机引物中筛选出8个有效引物，共扩增出69个可分析位点，其中多态位点65个，多态性务带比率（PPL）为94．20％。实验表明基因分化系数（Gst）平均值为0．0622，居群间的基因流（Nm）为7．5378，上述结果表明中国水仙居群的遗传变异大部分存在于居群内部，5个居群间的基因交流很高，种群间的分化很少。
본실험이용ISSR분자표기기술연구5개중국수선（Narcissus tazetta var．chinensis Boem＆Chen）거군적유전다양성。종40개수궤인물중사선출8개유효인물，공확증출69개가분석위점，기중다태위점65개，다태성무대비솔（PPL）위94．20％。실험표명기인분화계수（Gst）평균치위0．0622，거군간적기인류（Nm）위7．5378，상술결과표명중국수선거군적유전변이대부분존재우거군내부，5개거군간적기인교류흔고，충군간적분화흔소。
In this study, study on ISSR markers of five Chinese narcissus （Narcissus that tazetta var chinensis, the Boem ＆ Chen） population genetic diversity. The eight primers selected from 40 random primers amplified a total of 69 analyzed sites ofpolymorphic loci and 65, the percentage of polymorphic bands （PPL） is 94.20%. Experiments show that the average gene differentiation coefficient （Gst） was 0.0622, gene flow among populations （Nm） 7.5378, the above results show that Xianju group of Chinese water most of the genetic variation within populations, five genes among populations the exchange of high population differentiation.