CAJ | 학술논문

为保护野生资源,实现人工栽培,以大三叶升麻茎为试验材料,进行愈伤组织的培养,以及试管苗的生根、生根继代培养和试管苗的移栽、定植的研究,建立起野生大三叶升麻无性系。结果表明：MS＋BA 0.2 mg/L＋2,4-D 2.0～2.5 mg/L是嫩茎愈伤组织诱导培养的理想培养基;MS＋AgNO31.0 mg/L＋GA30.5 mg/L＋ZT 0.8 mg/L＋NAA 0.1 mg/L是嫩茎愈伤组织分化培养的理想培养基;把不定芽的基部在NAA 4.0mg/L的溶液中处理约5 min,接种到1/3MS为基本培养基,附加IBA 0.6 mg/L的培养基上,进行生根培养的方法是生根培养的理想培养方法;1/3MS＋IBA 0.6 mg/L＋NAA 0.1 mg/L也是增殖培养的理想培养基;试管苗移栽、定植易成活。定植成活的试管苗保持了野生大三叶升麻的所有植物学性状。
위보호야생자원,실현인공재배,이대삼협승마경위시험재료,진행유상조직적배양,이급시관묘적생근、생근계대배양화시관묘적이재、정식적연구,건립기야생대삼협승마무성계。결과표명：MS＋BA 0.2 mg/L＋2,4-D 2.0～2.5 mg/L시눈경유상조직유도배양적이상배양기;MS＋AgNO31.0 mg/L＋GA30.5 mg/L＋ZT 0.8 mg/L＋NAA 0.1 mg/L시눈경유상조직분화배양적이상배양기;파불정아적기부재NAA 4.0mg/L적용액중처리약5 min,접충도1/3MS위기본배양기,부가IBA 0.6 mg/L적배양기상,진행생근배양적방법시생근배양적이상배양방법;1/3MS＋IBA 0.6 mg/L＋NAA 0.1 mg/L야시증식배양적이상배양기;시관묘이재、정식역성활。정식성활적시관묘보지료야생대삼협승마적소유식물학성상。
For the protection of the wild resources and realization artificial cultivation,the tender stems of Cimicifuga simpiex were used as materials to do the study by using tissue culture methods.The study was mainly about callus induction and differentiation,adventitious buds rooting and rooting of the subculture,tube seedlings transplanting colonization,and establishment the clone of Cimicifuga simpiex.The results showed that MS＋BA 0.2 mg/L＋2,4-D 2.0～2.5 mg/L and MS＋AgNO3 1.0 mg/L＋GA3 0.5 mg/L＋ZT 0.8 mg/L＋NAA 0.1 mg/L were the optimum media for callus induction and differentiation respectively.After dealt with 4.0 mg/L NAA for 5 minutes,the adventitious buds could be induced rooting in the medium of 1/3MS＋IBA 0.6 mg/L.The ideal medium for rooting of the subculture was 1/3MS＋IBA 0.6 mg/L＋NAA 0.1 mg/L.The tube seedlings could be survived after transplanting colonization.The colonization of plantlets maintained all biological traits which wild plant has.