中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2013年
3期
350-352
,共3页
抗原,Thy-1%成纤维细胞%肺%细胞增殖%脂多糖类
抗原,Thy-1%成纖維細胞%肺%細胞增殖%脂多糖類
항원,Thy-1%성섬유세포%폐%세포증식%지다당류
Antigen,Thy-1%Fibroblast%Lung%Cell proliferation%Lipopolysaccharides
目的 评价脂多糖(LPS)对小鼠肺成纤维细胞胸腺细胞分化抗原-1(Thy-1)mRNA表达的影响.方法 原代培养小鼠肺成纤维细胞接种于96孔培养板密度(1×104/ml).培养48 h后,将其分为4组(n=3):PBS对照组(C组)、0.01 μg/ml LPS组(LPS0.01组)、0.10 μg/ml LPS组(LPS0.10组)和1.00μg/ml LPS组(LPS1.00组),分别加入PBS或以上终浓度LPS,在加入后即刻、6、24、48和72 h时(T0-4),分别采用CCK-8细胞计数法检测细胞增殖水平,RT-PCR法检测细胞Thy-1 mRNA表达.结果 与C组相比,T3,4时其余组细胞增殖水平升高,Thy-1 mRNA表达下调(P＜0.05)；T3,4时LPS0.01组、LPS0.10组和LPS1.00组细胞增殖水平依次升高,Thy-1 mRNA表达依次下调(P＜0.05).结论 LPS可下调Thy-1 mRNA表达,引起小鼠肺成纤维细胞异常增殖,提示内毒素血症诱发肺纤维化与肺成纤维细胞Thy-1表达下调有关.
目的 評價脂多糖(LPS)對小鼠肺成纖維細胞胸腺細胞分化抗原-1(Thy-1)mRNA錶達的影響.方法 原代培養小鼠肺成纖維細胞接種于96孔培養闆密度(1×104/ml).培養48 h後,將其分為4組(n=3):PBS對照組(C組)、0.01 μg/ml LPS組(LPS0.01組)、0.10 μg/ml LPS組(LPS0.10組)和1.00μg/ml LPS組(LPS1.00組),分彆加入PBS或以上終濃度LPS,在加入後即刻、6、24、48和72 h時(T0-4),分彆採用CCK-8細胞計數法檢測細胞增殖水平,RT-PCR法檢測細胞Thy-1 mRNA錶達.結果 與C組相比,T3,4時其餘組細胞增殖水平升高,Thy-1 mRNA錶達下調(P＜0.05)；T3,4時LPS0.01組、LPS0.10組和LPS1.00組細胞增殖水平依次升高,Thy-1 mRNA錶達依次下調(P＜0.05).結論 LPS可下調Thy-1 mRNA錶達,引起小鼠肺成纖維細胞異常增殖,提示內毒素血癥誘髮肺纖維化與肺成纖維細胞Thy-1錶達下調有關.
목적 평개지다당(LPS)대소서폐성섬유세포흉선세포분화항원-1(Thy-1)mRNA표체적영향.방법 원대배양소서폐성섬유세포접충우96공배양판밀도(1×104/ml).배양48 h후,장기분위4조(n=3):PBS대조조(C조)、0.01 μg/ml LPS조(LPS0.01조)、0.10 μg/ml LPS조(LPS0.10조)화1.00μg/ml LPS조(LPS1.00조),분별가입PBS혹이상종농도LPS,재가입후즉각、6、24、48화72 h시(T0-4),분별채용CCK-8세포계수법검측세포증식수평,RT-PCR법검측세포Thy-1 mRNA표체.결과 여C조상비,T3,4시기여조세포증식수평승고,Thy-1 mRNA표체하조(P＜0.05)；T3,4시LPS0.01조、LPS0.10조화LPS1.00조세포증식수평의차승고,Thy-1 mRNA표체의차하조(P＜0.05).결론 LPS가하조Thy-1 mRNA표체,인기소서폐성섬유세포이상증식,제시내독소혈증유발폐섬유화여폐성섬유세포Thy-1표체하조유관.
Objective To evaluate the effect of lipopolysaccharide (LPS) on thymocyte differentiation antigen-1 (Thy-1) mRNA expression in mouse lung fibroblasts.Methods Primary cultured mouse lung fibroblasts were seeded in 96-well plates with the density of 1 × 104/ml.After being cultured for 48 h,the cells were randomly divided into 4 groups (n =3 each):PBS control group (group C),LPS 0.01 μg/ml group (group LPS0.01),LPS 0.10 μg/ml group (group LPS0.10),and LPS 1.00 μg/ml group (group LPS1.00).PBS was added to the 96-well plates in group C.LPS with the final concentrations of 0.01,0.10 and 1.00 μg/ml were added to the 96-well plates in groups LPS0.01,LPS0.10 and LPS1.00,respectively.After being incubated for 0,6,24,48 and 72 h (T0-4),the proliferation of the cells was measured by CCK-8 assay and Thy-1 mRNA expression was detected by real-time PCR.Results Compared with group C,the proliferation of the cells was significantly increased,while Thy-1 mRNA expression was down-regulated at T3,4 in groups LPS0.01,LPS0.10 and LPS1.00 (P ＜ 0.05).The proliferation of the cells was gradually increased,while Thy-1 mRNA expression was gradually down-regulated in groups LPS0.01,LPS0.10 and LPS1.00 at T3,4 (P ＜ 0.05).Conclusion LPS results in abnormal proliferation of mouse lung fibroblasts through down-regulating Thy-1 mRNA expression,indicating that endoxemia-induced pulmonary fibrosis is related to the down-regulation of Thy-1 mRNA expression in lung fibroblasts.
