CAJ | 학술논문

目的：观察甲氨蝶呤对大鼠脊髓挫伤后脂质过氧化的影响，探讨其急性期抗氧化神经保护机制。方法：采用PinPointTM精密皮质撞击器制备大鼠脊髓挫伤模型，伤后30 min皮下注射（subcutaneous injection，sc）甲氨蝶呤（0.5 mg·kg-1·BW），采用酶联免疫吸附法检测血浆中丙二醛（malondialdehyde，MDA）和8-异前列腺素F2α（8-iso-Prostaglandin F2α，8-iso-PGF2α）的含量以及损伤组织中4-羟基壬烯醛-His加合物（4-hydroxynonenal-His adduct，HNE）的含量。结果：伤后1、3、6、12、24、48、72 h，甲氨蝶呤组MDA、8-iso-PGF2α以及HNE的含量均低于模型组；尤其在伤后6、12 h，甲氨蝶呤组血浆中MDA和脊髓组织中HNE含量均显著低于模型组（P<0.05）；在伤后12 h，血浆中8-iso-PGF2α含量也显著低于模型组（P<0.05）。结论：合适剂量的甲氨蝶呤防止脊髓继发性损伤可能与其抑制或减轻脂质过氧化有关。
목적：관찰갑안접령대대서척수좌상후지질과양화적영향，탐토기급성기항양화신경보호궤제。방법：채용PinPointTM정밀피질당격기제비대서척수좌상모형，상후30 min피하주사（subcutaneous injection，sc）갑안접령（0.5 mg·kg-1·BW），채용매련면역흡부법검측혈장중병이철（malondialdehyde，MDA）화8-이전렬선소F2α（8-iso-Prostaglandin F2α，8-iso-PGF2α）적함량이급손상조직중4-간기임희철-His가합물（4-hydroxynonenal-His adduct，HNE）적함량。결과：상후1、3、6、12、24、48、72 h，갑안접령조MDA、8-iso-PGF2α이급HNE적함량균저우모형조；우기재상후6、12 h，갑안접령조혈장중MDA화척수조직중HNE함량균현저저우모형조（P<0.05）；재상후12 h，혈장중8-iso-PGF2α함량야현저저우모형조（P<0.05）。결론：합괄제량적갑안접령방지척수계발성손상가능여기억제혹감경지질과양화유관。
Objective:This study examined the effect of methotrexate on acute phrase of spinal cord contusion-induced lipid peroxidation in rats and the neuroprotective mechanism of its anti-oxidant action. Methods:Rat spinal cord contusion model was prepared by Pinpoint Precision Cortical ImpactorTM apparatus and then methotrexate(0.5mg·kg-1·BW)was subcutaneously administrated at posttraumatic 30 min. ELISA method was used to determine the content of malondialdehyde(MDA)and 8-iso-PGF2αin plasma and 4-hydroxynonenal-His adduct (HNE)in injuried tissue. Results:Posttraumatic 1,3,6,12,24,48,72 h,the contents of MDA, 8-iso-PGF2αand HNE in the methotrexate group were all lower than those in the model group, which achieved statistical significance at posttraumatic 6,12 h(P<0.05). At posttraumatic 12 h, the content of 8-iso-PGF2αin plasma was also significantly lower than that in the model group (P<0.05). Conclusion:The appropriate doses,methotrexate could prevent secondary injury of spinal cord,which may be related to inhibiting or reducing the lipid peroxidation.